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Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array  Eva-Maria King, PhD, Lisa D. Vailes,

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Presentation on theme: "Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array  Eva-Maria King, PhD, Lisa D. Vailes,"— Presentation transcript:

1 Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array  Eva-Maria King, PhD, Lisa D. Vailes, MS, Amy Tsay, MSc, Shama M. Satinover, MS, Martin D. Chapman, PhD  Journal of Allergy and Clinical Immunology  Volume 120, Issue 5, Pages (November 2007) DOI: /j.jaci Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 A, Optimization of coupling concentration of IgE capture mAb for detection of total IgE. B, Comparison of total serum IgE levels measured by ImmunoCAP and multiplex; n = 63; ∗∗P < MFI, Median fluorescent intensity. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Specific IgE responses to indoor and outdoor allergens. Comparison between ELISA and multiplex, n = 95. ∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 A-D, Comparison of dust mite-specific IgE levels measured by different detection methods: multiplex, D. pteronyssinus (d1 CAP) and streptavidin ImmunoCAP, n = 106. ∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions


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