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Inflammatory cells, ceramides, and expression of proteases in perivascular adipose tissue adjacent to human abdominal aortic aneurysms  Maggie Folkesson,

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Presentation on theme: "Inflammatory cells, ceramides, and expression of proteases in perivascular adipose tissue adjacent to human abdominal aortic aneurysms  Maggie Folkesson,"— Presentation transcript:

1 Inflammatory cells, ceramides, and expression of proteases in perivascular adipose tissue adjacent to human abdominal aortic aneurysms  Maggie Folkesson, PhD, Emina Vorkapic, MSc, Erich Gulbins, MD, Lukasz Japtok, PhD, Burkhard Kleuser, PhD, Martin Welander, MD, Toste Länne, MD, Dick Wågsäter, PhD  Journal of Vascular Surgery  Volume 65, Issue 4, Pages e1 (April 2017) DOI: /j.jvs Copyright © 2016 The Authors Terms and Conditions

2 Fig 1 An open repair surgery where aneurysm sack is seen with some perivascular fat covering the enlarged aorta (black arrows) (A). Microscopic image of perivascular fat (arrow) seen in experimental model of aneurysm (B) and its control where no perivascular adipose tissue (PVAT) is seen (C). Similar to human abdominal aortic aneurysm (AAA), mouse models of AAA form PVAT something that is absent in control mice. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2016 The Authors Terms and Conditions

3 Fig 2 Immunohistochemistry. A, Perililpin; B, enlarged perilipin; C, CD66b; and D, CD68. The arrows show 3,3′-diaminobenzidine (DAB) brown positive staining in each image except in (B), which shows perilipin negative necrotic fat cells. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2016 The Authors Terms and Conditions

4 Fig 3 Presence of mast cell tryptase (A), T-cells (B), and matrix metalloproteinase (MMP)-9 (C) in perivascular adipose tissue (PVAT; arrows). Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2016 The Authors Terms and Conditions

5 Fig 4 Gene expression of perilipin (A), adiponectin (B), CD68 (C), CD3e (D), mast cell chymase (E), and CD66b (F) from human nonaneurysmal control aortas, abdominal aortic aneurysm (AAA) samples and periaortic fat as determined by real-time polymerase chain reaction (PCR) (n = 9 in all groups). *P < .05, **P < .01, ***P < .001.Open circle, ○ = Outlier greater than 1.5 times the interquartile range. Filled circle, ● = Outlier greater than 3 times the interquartile range. A.adv, Aortic adventitia; A.med, aortic intima/media; PVAT, perivascular adipose tissue. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2016 The Authors Terms and Conditions

6 Fig 5 Gene expression of interleukin (IL)-6 (A), caspase 3 (B), cathepsin K (C), Cathepsin L (D) and Cathepsin S (E) from human nonaneurysmal control aortas, abdominal aortic aneurysm (AAA) samples, and periaortic fat as determined by real-time polymerase chain reaction (PCR) (n = 9 in all groups). *P < .05. Open circle, ○ = Outlier greater than 1.5 times the interquartile range. Filled circle, ● = Outlier greater than 3 times the interquartile range. A.adv, Aortic adventitia; A.med, aortic intima/media; PVAT, perivascular adipose tissue. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2016 The Authors Terms and Conditions

7 Fig 6 Correlations between gene expression and ceramide/enzyme content of perivascular adipose tissue (PVAT). C16 and CD68 messenger RNA (mRNA) (A), C24:1 and CD66b mRNA (B), S1P and CD66b mRNA (C), and C16 and CD3 mRNA (D) (n = 6). Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2016 The Authors Terms and Conditions

8 Supplementary Fig (online only)
Gene expression of matrix metalloproteinase (MMP) 2 (A), MMP-8 (B), MMP-9 (C), MMP-12 (D), and tumor necrosis factor (TNF)-α (E) from human nonaneurysmal control aortas, abdominal aortic aneurysm (AAA) samples, and periaortic fat as determined by real-time polymerase chain reaction (PCR; n = 9 in all groups). *P < .05 and **P < .01. Open circle, ○ = Outlier greater than 1.5 times the interquartile range. Filled circle, ● = Outlier greater than 3 times the interquartile range. A.adv, Aortic adventitia; A.med, aortic intima/media; PVAT, perivascular adipose tissue. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2016 The Authors Terms and Conditions

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