1. Quiz#8 (8%) LC710 11/26/12 name___________
Q1:Draw a graph showing changes in GFP protein levels as consequences of
no Dox (at steady state) vs gradual addition of Dox (2% ea)
If Pax6 is a strong repressor
Pax6
tetO
minPr
Pax6
GFP
CMV
Pax6
minPr
tTA
If Pax6 is a activator
Pax6
tetO
minPr
Pax6
GFP
CMV
minPr
tTA

2. Q2:Draw a graph showing changes in GFP protein levels as consequences of
no Dox (at steady state) vs gradual addition of Dox (2% ea)
If Pax6 is a strong repressor
Pax6
tetO
minPr
Pax6
GFP
tetO
minPr
rtTA
If Pax6 is a activator
CMV
tetO
minPr
Pax6
GFP
Pax6
minPr
rtTA
EC: Why use Dox vs Tetracycline for these inducible systems in Eukaryotic cells? (1%)
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