1. Yeast Transformation
Mod 2 Day 2
Thursday Oct 9th

2. A little more about your yeast
NY411
MAT(A) his4-917d, lys2-173R2, leu2d1, ura3-52, trp1d63

3. A little more about your yeast
NY411
MAT(A) his4-917d, lys2-173R2, leu2d1, ura3-52, trp1d63
Ty917

4. A little more about your yeast
NY411
MAT(A) his4-917d, lys2-173R2, leu2d1, ura3-52, trp1d63

5. Transformation Wash the cells with Wash Sol’n (= water?)
Resuspend the cells in Competent Sol’n (= LiAc?)
Mix with your DNA samples
No DNA, pRS414, concentrated/cleaned PCR
Resuspend in Transformation Sol’n (= PEG?)
1 hr, 30°
Plate on SC-trp

6. Select TRP+ transformants

7. For next time…. Work on your materials and methods to this point
Read article for discussion in lab
Revise Mod1 report if you want
Enjoy the fall weekend…you’ve all been working SO hard