1. Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique 
Giuseppe Ricci, M.D., Sandra Perticarari, B.Sc., Rita Boscolo, B.Sc., Marcella Montico, B.Sc., Secondo Guaschino, M.D., Gianni Presani, B.Sc. 
Fertility and Sterility 
Volume 91, Issue 2, Pages (February 2009)
DOI: /j.fertnstert
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Cytofluorimetric analysis of dot-plot with log of green fluorescence (Syto16) versus log of red (7-AAD) fluorescence (A, B, C) displaying viable (R2), apoptotic (R3) and dead (R4) sperm cells. Plots D, E, F represent FSC versus SSC parameters on the basis of which a gate R5 sperm was set up to eliminate cell debris from analysis. Region R6 represents the bead population for absolute count. Cytograms A and D represent the analysis of semen sample before preparation, B and E after density-gradient centrifugation, C and F after swim-up technique.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions