1. Superoxide enhances interleukin 1β–mediated transcription of the hepatocyte-inducible nitric oxide synthase gene 
Paul C. Kuo, Keith Abe, Rebecca A. Schroeder 
Gastroenterology 
Volume 118, Issue 3, Pages (March 2000)
DOI: /S (00)70268-X
Copyright © 2000 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Nucleotide sequence of the rat hepatocyte iNOS promoter (1845 bp; GenBank X95629). ARE and NF-κB regulatory elements are labeled.
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Structure of successive 5'-deletion constructs of the rat hepatocyte iNOS promoter. Numbers assigned to the deletion plasmids correspond to the 5'-ends of the 5'-flanking sequence of the promoter from the transcription start site. Locations of the 2 NF-κB sites are labeled for all deletion constructs.
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Nuclear run-on assay of iNOS gene transcription in control, IL-1β–, and BZT-treated hepatocytes, as described in Materials and Methods. β-Actin and λ-phage cDNA served as positive and negative controls, respectively. Blot is representative of 4 experiments. IL-1β, 100 U/mL; BZT, 10 or 100 μmol/L.
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Nuclear run-on assay of iNOS gene transcription in control, IL-1β–, and BZT-treated hepatocytes in the presence and absence of SOD, as described in Materials and Methods. β-Actin and λ-phage cDNA served as positive and negative controls, respectively. Blot is representative of 4 experiments. IL-1β, 100 U/mL; BZT, 10 or 100 μmol/L; SOD, 100 U/mL.
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

6. Fig. 5
(A) iNOS promoter activity in rat hepatocytes stimulated with IL-1β and BZT. Total CAT expression was normalized to β-galactosidase activity and total protein. Data are expressed as mean ± SD of 4 experiments (ANOVA, P = for all concentrations of IL-1β; P < 0.05 IL-1β 10 U/mL vs. IL-1β 100 U/mL for all concentrations of BZT; P < 0.05 IL-1β 100 U/mL vs. IL-1β 1000 U/mL for all concentrations of BZT; P < 0.02 IL-1β 1000 U/mL vs. IL-1β 10 U/mL for all concentrations of BZT). (B) iNOS promoter activity in rat hepatocytes stimulated with IL-1β, BZT, and SOD (100 U/mL). Total CAT expression was normalized to β-galactosidase activity and total protein. Data are expressed as mean ± SD of 4 experiments (P < 0.05 IL-1β 10 U/mL + SOD vs. IL-1β 100 U/mL + SOD for all concentrations of BZT; P < 0.05 IL-1β 100 U/mL + SOD vs. IL-1β 1000 U/mL + SOD for all concentrations of BZT; P < 0.02 IL-1β 1000 U/mL + SOD vs. IL-1β 10 U/mL + SOD for all concentrations of BZT).
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

7. Fig. 5
(A) iNOS promoter activity in rat hepatocytes stimulated with IL-1β and BZT. Total CAT expression was normalized to β-galactosidase activity and total protein. Data are expressed as mean ± SD of 4 experiments (ANOVA, P = for all concentrations of IL-1β; P < 0.05 IL-1β 10 U/mL vs. IL-1β 100 U/mL for all concentrations of BZT; P < 0.05 IL-1β 100 U/mL vs. IL-1β 1000 U/mL for all concentrations of BZT; P < 0.02 IL-1β 1000 U/mL vs. IL-1β 10 U/mL for all concentrations of BZT). (B) iNOS promoter activity in rat hepatocytes stimulated with IL-1β, BZT, and SOD (100 U/mL). Total CAT expression was normalized to β-galactosidase activity and total protein. Data are expressed as mean ± SD of 4 experiments (P < 0.05 IL-1β 10 U/mL + SOD vs. IL-1β 100 U/mL + SOD for all concentrations of BZT; P < 0.05 IL-1β 100 U/mL + SOD vs. IL-1β 1000 U/mL + SOD for all concentrations of BZT; P < 0.02 IL-1β 1000 U/mL + SOD vs. IL-1β 10 U/mL + SOD for all concentrations of BZT).
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

8. Fig. 6
(A) Normalized CAT expression in rat hepatocytes transfected with iNOS promoter constructs and stimulated with IL-1β and BZT. Data are expressed as mean ± SD of 4 experiments. #P < 0.01 vs. control; *P < 0.01 vs. IL-1β. (B) Normalized CAT expression in rat hepatocytes transfected with iNOS promoter constructs and stimulated with IL-1β and BZT. Data are expressed as mean ± SD of 4 experiments. #P < 0.01 vs. control; *P < 0.01 vs. IL-1β.
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

9. Fig. 6
(A) Normalized CAT expression in rat hepatocytes transfected with iNOS promoter constructs and stimulated with IL-1β and BZT. Data are expressed as mean ± SD of 4 experiments. #P < 0.01 vs. control; *P < 0.01 vs. IL-1β. (B) Normalized CAT expression in rat hepatocytes transfected with iNOS promoter constructs and stimulated with IL-1β and BZT. Data are expressed as mean ± SD of 4 experiments. #P < 0.01 vs. control; *P < 0.01 vs. IL-1β.
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

10. Fig. 7
Schematic representation of oligonucleotides selected from the region nt 1359 to nt 1305 for use in gel shift assays. Each oligonucleotide has an overlap of 3–6 nucleotides.
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

11. Fig. 8
Gel shift analysis using ARE2, Redox 1, and Redox 2 in IL-1β (1000 U/mL)- and BZT (100 μmol/L)-stimulated rat hepatocytes. Representative of 3 analyses.
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

12. Fig. 9
Gel shift analysis using ARE2 and mARE2 in IL-1β (1000 U/mL)- and BZT (100 μmol/L)-stimulated rat hepatocytes. Representative of 3 analyses.
Gastroenterology  , DOI: ( /S (00)70268-X)
Copyright © 2000 American Gastroenterological Association Terms and Conditions