1. UNISEC Correlates of Protection for
Universal Influenza Vaccine Candidates
Problem 1: Correlates of CMI-induced immunity unknown
Solution: Measure as many as possible
Problem 2: Limited number of cell aliquots ava ilable
Solution: Simultaneous measurement
Problem 3: Interlaboratory standardisation of cell-based assays very difficult
Solution: Carry out core assay in one central laboratory
CMI
PBMC from vaccinated patients
stimulated by antigens/peptides
of choice for six hours in the
presence of co-stimulatory
antibodies and protein transport
inhibitors. Cells stained for
surface markers (CD3, CD4, CD8)
Cells stained for cytoplasmic
cytokines (IFNγ, IL2, IL4, TNFα)
before FACS analysis.
Other Assays
Collaborating partners:
UNISEC has been made possible
by contributions from the European
Commission DG-Research and the
European member states