Presentation is loading. Please wait.

Presentation is loading. Please wait.

Nick D. Tsihlis, PhD, Jozef Murar, BA, Muneera R. Kapadia, MD, Sadaf S

Published byHồ Hữu Modified over 5 years ago

Similar presentations

Presentation on theme: "Nick D. Tsihlis, PhD, Jozef Murar, BA, Muneera R. Kapadia, MD, Sadaf S"— Presentation transcript:

1 Isopropylamine NONOate (IPA/NO) moderates neointimal hyperplasia following vascular injury 
Nick D. Tsihlis, PhD, Jozef Murar, BA, Muneera R. Kapadia, MD, Sadaf S. Ahanchi, MD, Christopher S. Oustwani, BA, Joseph E. Saavedra, PhD, Larry K. Keefer, PhD, Melina R. Kibbe, MD  Journal of Vascular Surgery  Volume 51, Issue 5, Pages (May 2010) DOI: /j.jvs Copyright © Terms and Conditions

2 Fig 1 Isopropylamine NONOate (IPA/NO) prevents vascular smooth muscle cell (VSMC) proliferation in vitro, but does not cause cell death or affect migration. A, Proliferation of VSMC was assessed using 3H-thymidine incorporation after 24 hours of treatment with IPA/NO. B, VSMC death was assessed via Guava personal cell analysis (PCA) after 24 hours of treatment with IPA/NO. C, The effect of IPA/NO (1000 μmol/L) on VSMC migration was assessed by blinded counting of nuclei migrating into the scraped area. The effects of the parent compound, IPA, and breakdown product of IPA/NO, isopropanol, on proliferation, death and migration were also assessed at 1000 μmol/L. The effects of the NO donor S-nitroso-N-acetylpenicillamine (SNAP) on proliferation and cell death were assessed at 1000 μmol/L. *P < .005 vs control. †P < .05 vs 1000 μmol/L IPA/NO. n = 3/treatment group. Data shown are representative of three separate experiments. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

3 Fig 2 Isopropylamine NONOate (IPA/NO) prevents HUVEC proliferation in vitro, but does not cause cell death or affect migration. A, Proliferation of HUVEC was assessed using 3H-thymidine incorporation. B, HUVEC death was assessed via Guava personal cell analysis (PCA) after 24 hours of treatment with IPA/NO. C, The effect of IPA/NO (1000 μmol/L) on HUVEC migration was assessed by blinded counting of nuclei migrating into the scraped area. The effects of the parent compound, IPA, and breakdown product, isopropanol, of IPA/NO on proliferation, death and migration were also assessed at 1000 μmol/L. *P < .005 vs control. n = 3/treatment group. Data shown are representative of three separate experiments. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

4 Fig 3 Isopropylamine NONOate (IPA/NO) causes S-phase cell cycle arrest. A, Flow cytometry analysis of untreated and IPA/NO-treated (1000 μmol/L) vascular smooth muscle cell (VSMC). B, Graphical representation of the flow cytometry results after 24 hours of IPA/NO treatment. *P < .05 vs control. n = 3/treatment group. Data shown are representative of three separate experiments. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

5 Fig 4 Isopropylamine NONOate (IPA/NO) affects cell cycle protein expression, but not the phosphorylation state of cdks. A, The expression of cyclins, cdks, and CDKIs were assessed in vascular smooth muscle cell (VSMC) by Western blot analysis in the presence or absence of IPA/NO for 24 hours. Data shown are representative of three separate experiments. B, IPA/NO-treated and untreated VSMC lysates were immunoprecipitated (IP) with the indicated antibody, then analyzed for expression of phosphorylated (P) and non-phosphorylated (non-P) cdks. Graphs show the ratio of phosphorylated to non-phosphorylated protein as determined by densitometry. S, starved; C, control; and I, IPA/NO. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

6 Fig 5 Isopropylamine NONOate (IPA/NO) moderates neointimal hyperplasia in vivo. A, Balloon-injured rat carotid arteries treated with or without exogenous periadventitial administration of IPA/NO powder (10 mg) were harvested 14 days after injury and stained by H&E. Whole mount and close-up sections are shown. B, Quantitation of intimal area, medial area and arterial circumference using ImageJ software (n = 6/group). *P < .001 vs injury alone. Units are arbitrary. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

7 Fig 6 Isopropylamine NONOate (IPA/NO) decreases apoptosis in the neointima and media of balloon-injured arteries. Balloon-injured rat carotid arteries treated with or without exogenous periadventitial administration of IPA/NO powder (10 mg) and harvested 14 days after injury (n = 4/group) were subjected to TUNEL staining for apoptosis. Arrows indicate positive staining. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

8 Fig 7 Isopropylamine NONOate (IPA/NO) decreases inflammation in balloon-injured arteries. A, Balloon-injured rat carotid arteries treated with or without exogenous periadventitial administration of IPA/NO powder (10 mg) and harvested 14 days after injury (n = 6/group) were subjected to immunoperoxidase staining using antibodies against leukocytes (CD45) and macrophages (CD68). Arrows indicate positive staining. B, Blinded quantitation of positive CD45 and CD68 staining in untreated and IPA/NO-treated injured vessels was performed using ImageJ (n = 6/group). *P < .001 vs injury alone. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

9 Fig 8 Isopropylamine NONOate (IPA/NO) prevents re-endothelialization. A, Balloon-injured rats were administered Evans blue dye intravenously 7 days post-injury, and the harvested carotid arteries were photographed to assess the extent of endothelial regeneration. Blue staining indicates a lack of endothelium (n = 3/group). B, Quantitation of photographs from panel (A) using ImageJ software. *P = .001, n = 3/group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © Terms and Conditions

Download ppt "Nick D. Tsihlis, PhD, Jozef Murar, BA, Muneera R. Kapadia, MD, Sadaf S"

Similar presentations

Chrysin restores PDGF-induced inhibition on protein tyrosine phosphatase and reduces PDGF signaling in cultured VSMCs  Huey-Ming Lo, Min-Wen Wu, Shiow-Lin.

Chrysin restores PDGF-induced inhibition on protein tyrosine phosphatase and reduces PDGF signaling in cultured VSMCs  Huey-Ming Lo, Min-Wen Wu, Shiow-Lin.
Alpha-lipoic acid inhibits fractalkine expression and prevents neointimal hyperplasia after balloon injury in rat carotid artery  Kyeong-Min Lee, Keun-Gyu.

Alpha-lipoic acid inhibits fractalkine expression and prevents neointimal hyperplasia after balloon injury in rat carotid artery  Kyeong-Min Lee, Keun-Gyu.
Muneera R. Kapadia, MD, Lesley W. Chow, BS, Nick D

Muneera R. Kapadia, MD, Lesley W. Chow, BS, Nick D
Inhibition of intimal thickening after vascular injury with a cocktail of vascular endothelial growth factor and cyclic Arg-Gly-Asp peptide  Yue Li, Lucinda.

Inhibition of intimal thickening after vascular injury with a cocktail of vascular endothelial growth factor and cyclic Arg-Gly-Asp peptide  Yue Li, Lucinda.
High mobility group box 1 promotes endothelial cell angiogenic behavior in vitro and improves muscle perfusion in vivo in response to ischemic injury 

High mobility group box 1 promotes endothelial cell angiogenic behavior in vitro and improves muscle perfusion in vivo in response to ischemic injury 
Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 
Zinc finger protein 191 deficiency attenuates vascular smooth muscle cell proliferation, migration, and intimal hyperplasia after endovascular arterial.

Zinc finger protein 191 deficiency attenuates vascular smooth muscle cell proliferation, migration, and intimal hyperplasia after endovascular arterial.
Differential effects of orbital and laminar shear stress on endothelial cells  Alan Dardik, MD, PhD, Leiling Chen, MD, Jared Frattini, MD, Hidenori Asada,

Differential effects of orbital and laminar shear stress on endothelial cells  Alan Dardik, MD, PhD, Leiling Chen, MD, Jared Frattini, MD, Hidenori Asada,
Early hypercholesterolemia contributes to vasomotor dysfunction and injury associated atherogenesis that can be inhibited by nitric oxide  Kathleen G.

Early hypercholesterolemia contributes to vasomotor dysfunction and injury associated atherogenesis that can be inhibited by nitric oxide  Kathleen G.
An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb.

An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb.
Low molecular weight (LMW) heparin inhibits injury-induced femoral artery remodeling in mouse via upregulating CD44 expression  Gaofeng Zhao, MD, PhD,

Low molecular weight (LMW) heparin inhibits injury-induced femoral artery remodeling in mouse via upregulating CD44 expression  Gaofeng Zhao, MD, PhD,
Bertrand Poussier, MD, Alfredo C

Bertrand Poussier, MD, Alfredo C
Ezetimibe reduces intimal hyperplasia in rabbit jugular vein graft

Ezetimibe reduces intimal hyperplasia in rabbit jugular vein graft
Role of redox signaling and poly (adenosine diphosphate-ribose) polymerase activation in vascular smooth muscle cell growth inhibition by nitric oxide.

Role of redox signaling and poly (adenosine diphosphate-ribose) polymerase activation in vascular smooth muscle cell growth inhibition by nitric oxide.
Nitric oxide may inhibit neointimal hyperplasia by decreasing isopeptidase T levels and activity in the vasculature  Nick D. Tsihlis, PhD, Muneera R.

Nitric oxide may inhibit neointimal hyperplasia by decreasing isopeptidase T levels and activity in the vasculature  Nick D. Tsihlis, PhD, Muneera R.
Inhibitory effects of mesenchymal stem cells in intimal hyperplasia after balloon angioplasty  Ae-Kyeong Kim, PhD, Min-Hee Kim, Do-Hyung Kim, Ha-Nl Go,

Inhibitory effects of mesenchymal stem cells in intimal hyperplasia after balloon angioplasty  Ae-Kyeong Kim, PhD, Min-Hee Kim, Do-Hyung Kim, Ha-Nl Go,
Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries  Jens W Fischer,

Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries  Jens W Fischer,
VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery  Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang,

VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery  Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang,
Lysyl oxidase expression in a rat model of arterial balloon injury

Lysyl oxidase expression in a rat model of arterial balloon injury
Mechanical stress reduces podocyte proliferation in vitro

Mechanical stress reduces podocyte proliferation in vitro

Similar presentations

Ads by Google