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Efficacy of local nasal immunotherapy for Dp2-induced airway inflammation in mice: Using Dp2 peptide and fungal immunomodulatory peptide  Yi-Hsia Liu,

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Presentation on theme: "Efficacy of local nasal immunotherapy for Dp2-induced airway inflammation in mice: Using Dp2 peptide and fungal immunomodulatory peptide  Yi-Hsia Liu,"— Presentation transcript:

1 Efficacy of local nasal immunotherapy for Dp2-induced airway inflammation in mice: Using Dp2 peptide and fungal immunomodulatory peptide  Yi-Hsia Liu, MSc, Mei-Chen Kao, BS, Yih-Loong Lai, PhD, Jaw-Ji Tsai, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 112, Issue 2, Pages (August 2003) DOI: /mai Copyright © 2003 Mosby, Inc. Terms and Conditions

2 Fig. 1 Experimental schedule for intraperitoneal sensitization with rDp2 and local nasal immunotherapy with Dp2-derived peptides after immunization and subsequent intratracheal challenge with rDp2. Normal saline and dexamethasone were used as controls. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2003 Mosby, Inc. Terms and Conditions

3 Fig. 2 Effects of fungal immunomodulatory peptide, Dp , and Dp A on Dp2-specific IgG1 and IgG2a production in the sera. In IgG1, the serum was diluted 1:100 and IgG2a was diluted 1:5 at 4°C overnight. Detection antibodies were added separately for each assay, and the bound enzyme substrate was detected with TMB. The reaction was stopped with 50 μL 4N H2SO4 and measured at 450 nm. *P < .05 compared with normal saline. +P < .05 compared with naive. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2003 Mosby, Inc. Terms and Conditions

4 Fig. 3 Effects of fungal immunomodulatory peptide, Dp , and Dp A on the serial concentration of cytokines in sera. IFN-γ and IL-5 were measured using commercially available ELISA kits. The lowest range detectable was 10 pg/mL. For the protocols see “Methods.” *P < .05 compared with normal saline. +P < .05 compared with naive. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2003 Mosby, Inc. Terms and Conditions

5 Fig. 4 Effects of fungal immunomodulatory peptide, Dp , and Dp A on the serial concentration of cytokines in bronchoalveolar lavage fluid. *P < .05 compared with normal saline. +P < .05 compared with naive. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2003 Mosby, Inc. Terms and Conditions

6 Fig. 5 The effects of fungal immunomodulatory peptide, Dp , and Dp A on pulmonary function. The airway hypersensitivity to methacholine was measured both early and late after rDp2 IT challenge. The Penh values were accumulated after each dose of methacholine challenge and were expressed as the area under the curve.*P < .05 compared with normal saline. +P < .05 compared with naive. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2003 Mosby, Inc. Terms and Conditions

7 Fig. 6 The pathology of different groups of mice after local nasal immunotherapy. The lung tissues were obtained 2 days after intrathecal rDp2. A, Naive mice. B, Mice treated with normal saline. C, Mice treated with dexamethasone. D, Mice treated with fungal immunomodulatory peptide (FIP). E, Mice treated with Dp F, Mice treated with Dp A. G, Mice treated with FIP mixed with Dp H, Mice treated with FIP mixed with Dp A (hematoxylin-eosin stain, original magnification ×400). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2003 Mosby, Inc. Terms and Conditions

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