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Macrophage-Targeted Indocyanine Green-Neomannosyl Human Serum Albumin for Intraoperative Sentinel Lymph Node Mapping in Porcine Esophagus  Hyun Koo Kim,

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Presentation on theme: "Macrophage-Targeted Indocyanine Green-Neomannosyl Human Serum Albumin for Intraoperative Sentinel Lymph Node Mapping in Porcine Esophagus  Hyun Koo Kim,"— Presentation transcript:

1 Macrophage-Targeted Indocyanine Green-Neomannosyl Human Serum Albumin for Intraoperative Sentinel Lymph Node Mapping in Porcine Esophagus  Hyun Koo Kim, MD, PhD, Yu Hua Quan, PhD, Yujin Oh, MS, Ji Yong Park, MS, Ji-Ho Park, PhD, Yeonho Choi, PhD, Yun-Sang Lee, PhD, Jae Min Jeong, PhD, Young Ho Choi, MD, PhD, Beop-Min Kim, PhD  The Annals of Thoracic Surgery  Volume 102, Issue 4, Pages (October 2016) DOI: /j.athoracsur Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

2 Fig 1 Indocyanine green-neomannosyl human serum albumin (ICG:MSA) selectively enhances the fluorescence signal of cluster of differentiation (CD206)–expressed U937 cells. (A) U937 monocytic cells were differentiated by 12-O-tetradecanoylphorbol-13-acetate (PMA) treatment, and immunofluorescence staining was performed. The CD206 receptor was stained with CD206 antibody and the nucleus was stained with mounting medium with 4',6-diamidino-2-phenylindole (DAPI). The images of the differentiated U937 cells were analyzed using confocal microscopy. (B) The differentiated U937 cells were treated with ICG, ICG:human serum albumin (HSA) or ICG:MSA for 30 min, and the fluorescent signal was detected by an in vivo imaging system. The t test analysis resulted in statistically significant differences. *p < **p < The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

3 Fig 2 Body distribution of indocyanine green (ICG) or ICG:neomannosyl human serum albumin (ICG:MSA) in subcutaneously injected nude mice. ICG or ICG:MSA was subcutaneously injected in the footpads of nude mice, and the time-dependent distribution was investigated using an in vivo imaging system. ICG quickly spread throughout the body. However, ICG:MSA remained primarily in the popliteal lymph node. The injection site is shown by white arrows, the sentinel lymph node (SLN) is shown by a white circle, and a second lymph node (LN) is shown by a white dot circle. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

4 Fig 3 Schematic diagram of sentinel lymph node (SLN) mapping under intraoperative color and fluorescence-merged imaging system in porcine esophagus. The indocyanine green-neomannosyl human serum albumin (ICG:MSA) was injected into the submucosal layer of the midthoracic esophagus using an esophagoscope. The imaging system was composed of 2 cameras and a near-infrared light-emitting diode light source. The signal contrast of the fluorescence image was emphasized and merged with the color image, and the SLN and injection sites are shown as pseudocolored images on the monitor. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

5 Fig 4 Real-time intraoperative color and fluorescence-merged imaging system (ICFIS)–guided porcine esophageal sentinel lymph node (SLN) mapping by indocyanine green-neomannosyl human serum albumin (ICG:MSA). (A) Esophagoscopic and operative views of submucosal ICG:MSA injection of porcine esophagus. (B) The ICG:MSA submucosal location was confirmed on cross-sectioning of the injection site in the esophagus. (C) Fluorescent imaging of SLN around the injection site. (D) Fluorescent image-guided resection of SLN, ex vivo fluorescent image of SLN and non-SLN after resection, and SLN on hematoxylin and eosin (H&E) stain. The injection site is indicated by white arrows and the SLN is shown by a white circle. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

6 Supplemental Figure 1 The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

7 Supplemental Figure 2 The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2016 The Society of Thoracic Surgeons Terms and Conditions

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