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Aim #12: What are some other tools biologists use in the laboratory?
Date: September 29, 2016 Aim #12: What are some other tools biologists use in the laboratory? HW: Characteristics of Life Worksheet Castle Learning due Wednesday Unit 1 Test Thursday (p.1) & Friday (p.5) Do Now: How many micrometers are in 1 millimeter? Which part of the microscope controls the amount of light passing through the specimen? How do you calculate total magnification? Which parts of the microscope do you use to focus a specimen?
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1. How do we prepare a wet-mount?
Place drop of water on slide Place specimen in water Place cover slip on a 45º angle to avoid air bubbles
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2. What is an Electron Microscope?
Uses beams of electrons (NOT light waves) to produce magnified image specially fixed and treated and mounted in a vacuum
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How do they improve images?
High magnification Greater than 100,000X High resolution: the ability to separate two objects close together
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3. What is a stereoscope? AKA: Dissecting Microscope
Binocular: two tubes Presents a slightly different view of specimen to each eye… 3D Useful for opaque objects and small organisms or body structures
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4. What is a centrifuge? AKA: ultracentrifuge
Separates according to different densities by spinning sample in a test tube at very high speeds
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5. Why do we stain cells? Cell Structures can be made visible by treatment with solutions that color certain parts of cells and not others
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6. Why do we use indicators?
Substances that change color when in the presence of certain chemicals
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