1. Volume 143, Issue 6, Pages 1555-1563.e2 (December 2012)
Hepatic Vascular Endothelial Growth Factor Regulates Recruitment of Rat Liver Sinusoidal Endothelial Cell Progenitor Cells 
Lin Wang, Xiangdong Wang, Lei Wang, Jenny D. Chiu, Gijs van de Ven, William A. Gaarde, Laurie D. DeLeve 
Gastroenterology 
Volume 143, Issue 6, Pages e2 (December 2012)
DOI: /j.gastro
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2. Figure 1
Acute liver injury induced by DMN. (A) H&E stain of control liver and of liver 12–48 hours after DMN injection. (B) CD31 (FITC, green) immunostaining of control liver and of liver 24 hours after DMN treatment.
Gastroenterology  , e2DOI: ( /j.gastro )
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3. Figure 2
Proliferation, mobilization, and engraftment of BM SPCs after DMN injection. (A) BM SPC proliferation. The percentage of PCNA+ SPCs in the bone marrow in control rats and 1 day after DMN (n = 3; **P < .01). (B) Number of SPCs in BM. The number of SPCs in BM on day 1 after DMN injection (n = 3; **P < .01). (C) Mobilization of BM SPCs. The number of circulating BM SPCs (PB SPC) on day 1 after DMN injection (n = 3; **P < .01). PB SPC, SPC in peripheral blood. (D) Engraftment of BM SPCs. Representative example of FACS analysis showing the percentage GFP+ LSECs 5 days after DMN injection in a wild-type rat that had received a bone marrow transplantation from a Lew-Tg(CAG-EGFP)ys rat. Mean ± standard error of the mean of this group was 40.6% ± 2.1% GFP+ LSECs on day 5. (E) Engraftment of BM SPCs. Engraftment in the sinusoid of bone marrow–derived GFP+ SPCs on DMN day 5 is shown by immunostaining for CD31 (magenta), GFP (green), and the overlay (white) showing co-localization.
Gastroenterology  , e2DOI: ( /j.gastro )
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4. Figure 3
Effect of allogeneic, resident SPC infusion after DMN treatment. Rats received either an infusion of phosphate-buffered saline (PBS; top row) or 1 × 106 allogeneic, resident SPCs (bottom row) on day 1 after DMN and were killed on day 3. Compared with the PBS control, in rats that received an infusion of resident SPCs, (A) whole liver showed decreased hemorrhage, (B) histology showed decreased hemorrhage, congestion, and necrosis, and (C) CD31 staining (red) showed a marked increase in engrafted LSECs.
Gastroenterology  , e2DOI: ( /j.gastro )
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5. Figure 4
VEGF gene and protein expression. (A–C) Increased VEGF expression 1 day after DMN and (D–F) knockdown of VEGF expression by ASO. (A) Hepatic VEGF messenger RNA (mRNA) expression determined by real-time PCR in control and 1 day after DMN (n = 3; *P < .05). (B) Hepatic VEGF protein expression examined by immunoblot in control and 1 day after DMN. (C) Quantification of immunoblot in panel B (n = 3; ***P < .0001). (D) Hepatic VEGF mRNA expression determined by real-time PCR after control ASO and VEGF ASO knockdown (n = 3; *P < .05). (E) Hepatic VEGF protein expression examined by immunoblot after control ASO and VEGF ASO knockdown. (F) Quantification of immunoblot in panel E (n = 3; *P < .05).
Gastroenterology  , e2DOI: ( /j.gastro )
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6. Figure 5
Effect of VEGF knockdown on BM SPC proliferation and mobilization. Rats received scrambled control ASO or VEGF ASO for 4 weeks, were treated with DMN, and then killed on day 1 after DMN. (A) Proliferation of BM SPCs. The percentage of PCNA+ SPCs in the bone marrow (n = 3). (B) Number of SPCs in the bone marrow (n = 4). (C) Mobilization of BM SPCs. The number of BM SPCs in the circulation (n = 4). **P < .01 compared with control.
Gastroenterology  , e2DOI: ( /j.gastro )
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7. Figure 6
Effect of VEGF knockdown on engraftment and differentiation of BM SPCs. Wild-type rats received a bone marrow transplantation from a Lew-Tg(CAG-EGFP)ys, were treated 2 months later with scrambled control ASO or VEGF ASO for 4 weeks, and then were injected with DMN. (A) Engraftment of BM SPCs. Percentage of GFP+ LSECs was determined 14 days after DMN (n = 3; **P < .01). (B) Differentiation of engrafted SPCs. GFP+ LSECs were isolated 14 days after DMN and examined by scanning electron microscopy for the presence of fenestrae. Circles indicate sieve plates. Scale bar: 5 μm.
Gastroenterology  , e2DOI: ( /j.gastro )
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8. Figure 7
Effect of VEGF ASO knockdown on DMN injury. Rats were treated with scrambled control ASO or VEGF ASO for 4 weeks and killed on day 1 after DMN. (A) H&E staining. (B and C) Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (n = 3; *P < .01, **P < .001).
Gastroenterology  , e2DOI: ( /j.gastro )
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9. Supplementary Figure 1
Histology of DMN liver toxicity. H&E stain of (A) control liver, and (B) on day 3 and (C) day 5 after DMN administration.
Gastroenterology  , e2DOI: ( /j.gastro )
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10. Supplementary Figure 2
Bone marrow VEGF after VEGF ASO and scrambled ASO. (A) Immunoblot for VEGF after 4 weeks of ASO treatment and (B) densitometry of immunoblot (n = 3). There is no significant difference in bone marrow VEGF after scrambled ASO vs VEGF ASO.
Gastroenterology  , e2DOI: ( /j.gastro )
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11. Supplementary Figure 3
Engraftment of infused SPCs after partial hepatectomy. Wild-type Lewis rats were treated with scrambled ASO or VEGF ASO for 4 weeks, underwent partial hepatectomy, received an infusion on day 1 of GFP+ resident SPCs isolated from the livers of Lew-Tg(CAG-EGFP)ys rats. The percentage of GFP+ LSECs was determined 14 days after partial hepatectomy (n = 4; ***P < .0001).
Gastroenterology  , e2DOI: ( /j.gastro )
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