1. A B
Figure 1.

2. A B Figure 2. Day 4 Day 8 ALP activity (nmol/well) Control
Osteogenic media (OM)
Propofol (mM) 20 50 B
Control OM
OM + Propofol 5 mM
OM + Propofol 20 mM
OM + Propofol 50 mM
ALP activity (nmol/well)
N.S.
Figure 2.

3. A B * Figure 3. RT-PCR Quantitative Rea ltime-PCR ALP OCN OPN RANKL
OPG
HPRT
Propofol (mM) 20 50
Control
Osteogenic media (OM) B
Quantitative Rea ltime-PCR
Relative Exp./HPRT mRNA
Propofol (mM) 20 50
Control
Osteogenic media (OM) *
Figure 3.

4. (without M-CSF & RANKL)
Conditioned medium (C.M.) preparation from osteoblasts
Day 0
Day 7
Day 8
Osteogenic media (OM)
with or without propofol
Media
change
C.M.
Osteoclast differentiation analyses
Day 0
(BMM)
M-CSF & RANKL
TRAP
staining
C.M. treatment
(without M-CSF & RANKL)
Day 1
Day 2
(pre-OC)
Day 3
Day 4
Day 5
(OC) B
C.M.
Control
OM only
OM +
propofol 20 mM
propofol 50 mM
TRAP staining
(day 5)
OC number/well *
Figure 4.