Presentation is loading. Please wait.

Presentation is loading. Please wait.

Intraobserver and interobserver variability and spatial differences in histologic examination of carotid endarterectomy specimens  Willem E. Hellings,

Published byAsher Collins Modified over 5 years ago

Similar presentations

Presentation on theme: "Intraobserver and interobserver variability and spatial differences in histologic examination of carotid endarterectomy specimens  Willem E. Hellings,"— Presentation transcript:

1 Intraobserver and interobserver variability and spatial differences in histologic examination of carotid endarterectomy specimens  Willem E. Hellings, MD, Gerard Pasterkamp, MD, PhD, Anne Vollebregt, Cees A. Seldenrijk, MD, PhD, Jean-Paul P.M. De Vries, MD, PhD, Evelyn Velema, BSc, Dominique P.V. De Kleijn, PhD, Frans L. Moll, MD, PhD  Journal of Vascular Surgery  Volume 46, Issue 6, Pages (December 2007) DOI: /j.jvs Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 In this schematic representation of plaque segmentation, each transverse segment measures approximately 0.5 cm. The 0 segment, the reference segment that is routinely used for assessment of plaque histology in the Athero-Express study, is defined as the segment with greatest plaque burden. The adjacent segments at both sides are called +1, +2 etc, at one side and −1, −2, etc, at the other side. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Representative carotid plaque histology. Images A-G were photographed at ×20 original magnification. A, Hematoxylin and eosin (H&E) staining shows a plaque with no lipid core. B, H&E staining, large lipid core (L, lumen; C, core). The inset shows cholesterol crystals, which were clearly visible at higher magnification. C, Immunohistochemistry analysis with CD68 shows heavy macrophage infiltration. D, Immunohistochemistry analysis with α-actin shows heavy smooth muscle cell infiltration. E, Picrosirius red shows heavy collagen staining. F, Hematoxylin stains heavy calcification (blue). G, H&E, thrombus (arrows). H, At higher magnification (H&E, ×200 original magnification), signs of organization of the thrombus can be appreciated: angiomatosis (arrows) and influx of inflammatory cells. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Semiquantitative vs computer-aided measurements. Comparison of semiquantitative assessments, expressed as “no,” “minor,” “moderate,” and “heavy” (x-axis) and the computer-aided measurements, expressed as the percentage of plaque area occupied by the specific immunohistochemical staining in three representative fields (y-axis). A, Macrophage infiltration (CD68+). B, Smooth muscle cell infiltration (α-actin+). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Intersegment variability of histologic examination of the plaque. The similarity between different segments expressed as percentage of the κ value of the intraobserver repeatability, which is the theoretic maximum when two adjacent segments are completely equal: 100% indicates perfect similarity, 0% indicates no similarity, and negative values (not observed) indicate inverse associations. The similarity is compared between adjacent segments (intersegment distance “1”) and between the reference segment and a nonadjacent segment (intersegment distance “2”). MO, Macrophage infiltration; SMC, smooth muscle cell infiltration; coll, collagen; calc, calcifications; thro, thrombus; overall, overall phenotype. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

Download ppt "Intraobserver and interobserver variability and spatial differences in histologic examination of carotid endarterectomy specimens  Willem E. Hellings,"

Similar presentations

S. Shaikh, J. Brittenden, R. Lahiri, P. A. J. Brown, F. Thies, H. M

S. Shaikh, J. Brittenden, R. Lahiri, P. A. J. Brown, F. Thies, H. M
Feasibility of wall stress analysis of abdominal aortic aneurysms using three- dimensional ultrasound  Annette M. Kok, MSc, V. Lai Nguyen, MD, Lambert.

Feasibility of wall stress analysis of abdominal aortic aneurysms using three- dimensional ultrasound  Annette M. Kok, MSc, V. Lai Nguyen, MD, Lambert.
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,

In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,
Closure of the arteriotomy after carotid endarterectomy: Patch type is related to intraoperative microemboli and restenosis rate  Bart A.N. Verhoeven,

Closure of the arteriotomy after carotid endarterectomy: Patch type is related to intraoperative microemboli and restenosis rate  Bart A.N. Verhoeven,
Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS,

Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS,
Increased 18F-fluorodeoxyglucose uptake in abdominal aortic aneurysms in positron emission/computed tomography is associated with inflammation, aortic.

Increased 18F-fluorodeoxyglucose uptake in abdominal aortic aneurysms in positron emission/computed tomography is associated with inflammation, aortic.
Willem E. Hellings, MD, Gerard Pasterkamp, MD, PhD, Bart A. N

Willem E. Hellings, MD, Gerard Pasterkamp, MD, PhD, Bart A. N
Intraluminal abdominal aortic aneurysm thrombus is associated with disruption of wall integrity  Dave Koole, MD, Herman J.A. Zandvoort, MD, Arjan Schoneveld,

Intraluminal abdominal aortic aneurysm thrombus is associated with disruption of wall integrity  Dave Koole, MD, Herman J.A. Zandvoort, MD, Arjan Schoneveld,
Free-floating thrombus of the carotid artery: Literature review and case reports  Ahmad F. Bhatti, MD, Luis R. Leon, MD, RVT, Nicos Labropoulos, MD, Tara.

Free-floating thrombus of the carotid artery: Literature review and case reports  Ahmad F. Bhatti, MD, Luis R. Leon, MD, RVT, Nicos Labropoulos, MD, Tara.
Popliteal artery aneurysms differ from abdominal aortic aneurysms in cellular topography and inflammatory markers  Rob Hurks, MD, PhD, Rogier H.J. Kropman,

Popliteal artery aneurysms differ from abdominal aortic aneurysms in cellular topography and inflammatory markers  Rob Hurks, MD, PhD, Rogier H.J. Kropman,
Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.
Central venous catheter failure is induced by injury and can be prevented by stabilizing the catheter tip  Ted R. Kohler, MD, Thomas R. Kirkman, BS  Journal.

Central venous catheter failure is induced by injury and can be prevented by stabilizing the catheter tip  Ted R. Kohler, MD, Thomas R. Kirkman, BS  Journal.
Oral bacteria in the occluded arteries of patients with Buerger disease  Takehisa Iwai, MD, PhD, Yoshinori Inoue, MD, PhD, Makoto Umeda, DDS, PhD, Yi Huang,

Oral bacteria in the occluded arteries of patients with Buerger disease  Takehisa Iwai, MD, PhD, Yoshinori Inoue, MD, PhD, Makoto Umeda, DDS, PhD, Yi Huang,
Markers of instability in high-risk carotid plaques are reduced by statins  Hagen Kunte, MD, Nicola Amberger, MD, Markus Alexander Busch, MD, MPH, Ralph-Ingo.

Markers of instability in high-risk carotid plaques are reduced by statins  Hagen Kunte, MD, Nicola Amberger, MD, Markus Alexander Busch, MD, MPH, Ralph-Ingo.
Feasibility of wall stress analysis of abdominal aortic aneurysms using three- dimensional ultrasound  Annette M. Kok, MSc, V. Lai Nguyen, MD, Lambert.

Feasibility of wall stress analysis of abdominal aortic aneurysms using three- dimensional ultrasound  Annette M. Kok, MSc, V. Lai Nguyen, MD, Lambert.
Matrix metalloproteinase inhibition reduces intimal hyperplasia in a porcine arteriovenous-graft model  Joris I Rotmans, MD, Evelyn Velema, BSc, Hence.

Matrix metalloproteinase inhibition reduces intimal hyperplasia in a porcine arteriovenous-graft model  Joris I Rotmans, MD, Evelyn Velema, BSc, Hence.
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
Lucas P. Neff, MD, Bryan W. Tillman, MD, PhD, Saami K

Lucas P. Neff, MD, Bryan W. Tillman, MD, PhD, Saami K
Increased vascularization of shoulder regions of carotid atherosclerotic plaques from patients with diabetes  Fredrik J. Olson, PhD, Sofia Strömberg,

Increased vascularization of shoulder regions of carotid atherosclerotic plaques from patients with diabetes  Fredrik J. Olson, PhD, Sofia Strömberg,

Similar presentations

Ads by Google