1. Early- and Late-Stage Kaposi's Sarcoma-Derived Cells But Not Activated Endothelial Cells Can Invade De-Epidermized Dermis 
Thierry Simonart, Chantal Degraef, Roger Mosselmans, Philippe Hermans, Yanto Lunardi-Iskandar, Jean-Christophe Noel, Jean-Paul Van Vooren, Dominique Parent, Michel Heenen, Paul Galand 
Journal of Investigative Dermatology 
Volume 116, Issue 5, Pages (May 2001)
DOI: /j x
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Invasion of DED by KS cells but not by activated endothelial cells. (a) KS-2 cells were seeded at 1 × 106 cells in a volume of 50 µl on the surface of DED. One month after the plating, the samples were formalin fixed. Spindle-shaped cells are mainly located near the margin and are scattered between the collagen bundles. Note the absence of differentiation in vascular structures. (b) KS-Y1 cells were seeded at 1 × 106 cells in a volume of 50 µl on the surface of DED. One month after the plating, the samples were formalin fixed. By contrast to KS-derived cells, the KS-Y1 cells are more dense and display an epithelium-like differentiation on the surface of the dermis. (c) KS cell supernatant-activated HDMEC were seeded at 1 × 106 cells in a volume of 50 µl on the surface of DED. Presence of HDMEC on the surface of DED 3 d after the plating, suggesting that a loose adherence alone cannot explain the absence of invasion of the dermis by endothelial cells. (d) Fibroblasts were seeded at 1 × 106 cells in a volume of 50 µl on the surface of DED. One month after the plating, the samples were formalin fixed. These cells did not invade the DED. (e) Smooth muscle cells were seeded at 1 × 106 cells in a volume of 50 µl on the surface of DED. One month after the plating, the samples were formalin fixed. These cells did not invade the DED. Scale bar: 100 µM.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Time-dependent accumulation of KS cells in DED. KS-2 cells (▵) and KS-Y1 cells (•) were seeded at 1 × 106 cells in a volume of 50 µl on the surface of 1 cm2 DED. After various periods of incubation, the samples were formalin fixed. The number of cells located below the margin was counted in five randomly chosen microscopic fields at original magnification, × 250. The results are expressed as the mean ± SEM of the cell number from three independent experiments.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Staining of the invading KS-derived spindle cells with an anti-92 kDa type IV collagenase monoclonal antibody. Immunoperoxidase staining: brown reaction signifies positive detection of the specific antigen. (a) Control isotype. (b) Detection of the 92 kDa type IV collagenase in the cytoplasm of the cells. Scale bar: 40 µM.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions