1. Donor kidney biopsies: pathology matters, and so does the pathologist
Mark Haas 
Kidney International 
Volume 85, Issue 5, Pages (May 2014)
DOI: /ki
Copyright © 2014 International Society of Nephrology Terms and Conditions

2. Figure 1
Influence of paraffin versus frozen section and use of special stains on the ease of renal biopsy interpretation. All panels are from a pre-implantation needle core biopsy of an expanded-criteria donor who was known to have hypertension and diabetes, but a normal pre- procurement serum creatinine. A portion of the biopsy was processed for frozen section and stained with hematoxylin and eosin (H&E), and the remainder fixed in formalin and processed for paraffin sections that were stained with H&E and periodic acid–Schiff (PAS) stains. (a) Frozen section. There is hyaline arteriolosclerosis and an increase in mesangial matrix; however, these changes are subtle and could be missed by an inexperienced pathologist. The interstitium appears widened in comparison with the paraffin sections (b, c), a typical artifact seen in frozen sections. (b) H&E-stained paraffin section. In comparison with the frozen section, the increased mesangial matrix and arteriolar hyalinosis (seen to involve both afferent and efferent arterioles in this sample with diabetic nephropathy) are more easily discerned. (c) PAS-stained paraffin section. This is the section cut directly following that in (b), and the same microscopic field is shown. The PAS stain nicely highlights the increased mesangial matrix and arteriolar hyalinosis, and these changes are easily identified. The PAS staining of the tubular basement membranes allows for a more accurate estimation of the amount of interstitial tissue compared with the H&E stain (b). Original magnification of all three panels, × 200.
Kidney International  , DOI: ( /ki )
Copyright © 2014 International Society of Nephrology Terms and Conditions