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Teratogenic effects of retinoic acid on neurulation in mice embryos

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Presentation on theme: "Teratogenic effects of retinoic acid on neurulation in mice embryos"— Presentation transcript:

1 Teratogenic effects of retinoic acid on neurulation in mice embryos
M. Nobakht, A. Zirak, M. Mehdizadeh, P. Tabatabaeei  Pathophysiology  Volume 13, Issue 1, Pages (February 2006) DOI: /j.pathophys Copyright © Terms and Conditions

2 Fig. 1 Appearance of embryos of mice dams, which have received RA (from left to right): 40 and 60mg/kg on the eighth day of gestation and control. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

3 Fig. 2 The embryo from a dam having received 40mg/kg RA. The arrow indicates head cleft. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

4 Fig. 3 The embryo from a dam having received 60mg/kg RA. Arrow indicates the prolonged cranium and the small arrow indicates facial cleft. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

5 Fig. 4 Transverse paraffin section of closure neural tube of 10-day old embryo of a dam exposed to DMSO. Lumen (L), bar line=300μm. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

6 Fig. 5 Transverse epon section of neural tube of 10-day old embryo from a dam exposed to DMSO. Nucleoli (light arrow), mitosis (dark arrow), bar line=50μm. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

7 Fig. 6 Transverse paraffin section of non-closure (arrow) neural tube of 10-day old embryo of a dam exposed to 40mg/kg RA on the eighth day of gestation: ganglionic spinal (Gs), bar line=300μm. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

8 Fig. 7 Transverse paraffin section of neural tube of 10-day old embryo of a dam exposed to 40mg/kg RA on the eighth day of gestation. The light arrow indicates disorganization of neuroepitbelium and the dark arrow indicates closure neural tube, bar line=100μm. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

9 Fig. 8 Transverse epon section of neural tube of 10-day old embryo of a dam exposed to 40mg/kg RA on the eighth day of gestation. The density of chromatin and the number of nucleoli were decreased: venteral zone (Vz); mantle zone (Maz); marginal zone (Mrz), bar line=100μm. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

10 Fig. 9 Transverse paraffin section of non-closure (arrow) neural tube of 10-day old embryo from a dam exposed to 60mg/kg RA on the eighth days of gestation: lumen (L); ganglionic spinal (Gs), bar line=300μm. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

11 Fig. 10 Transverse epon section of the neural tube of 10-day old embryo exposed to 60mg/kg RA on the eighth day of gestation heterochromatin (light arrow), mitosis (dark arrow), bar line=50μm. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

12 Fig. 11 The comparison of longitudinal, medial and lateral measures (in μm) of the neural tube, embryos of control and RA exposed (on the eighth day of gestation) dams on the 10th day of gestation. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions

13 Fig. 12 The comparison of thickness of ventricular, mantle and marginal layers (in μm) of the neural tube embryos of control and RA exposed (on the eighth day) dams on the 10th day of gestation. Pathophysiology  , 57-61DOI: ( /j.pathophys ) Copyright © Terms and Conditions


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