1. Nanomechanical characterization of the NE.
Nanomechanical characterization of the NE. (A) Two images from the same sample showing the cytoplasmic side of the NE (top row), with the effective elastic moduli (Eeff)—determined from Hertz fits to individual force curves captured during imaging (bottom row; force curve frequency: 2 kHz). (B) As (A), for the nucleoplasmic side of the membrane, highlighting the NPC scaffolds and the lamina network as local enhancements in height and Eeff, and NPC baskets as local increases in height and as reductions in Eeff. (C) Cropped pores from (A), highlighting the variability in transport channel Eeff values for NPCs with similar topographies. Force at maximum indentation (Fmax, ±10% confidence interval): 397 pN (A) and 300 pN (B). Scale bar for all images (see A and C, top right panels): 100 nm. Colour scales: 70 nm and 6 MPa (A and C); 75 nm and 3 MPa (B).
George J Stanley et al. LSA 2018;1:e
© 2018 Stanley et al.