1. Co-stimulated/Tc2 cells abrogate murine marrow graft rejection
Andreas A. Erdmann, Unsu Jung, Jason E. Foley, Yoko Toda, Daniel H. Fowler 
Biology of Blood and Marrow Transplantation 
Volume 10, Issue 9, Pages (September 2004)
DOI: /j.bbmt

2. Figure 1
Characterization of in vitro-generated donor Th1/Tc1 (T1) and Th2/Tc2 (T2) cells. A, T-cell expansion and activation. Donor T cells were stimulated with anti-CD3/anti-CD28 beads in type 1 or type 2 culture conditions. Data shown are -fold increase in T-cell number (left panel) and change in T-cell activation, as measured by median cell volume (femtoliters; right panel); data are mean ± SEM of 3 experiments. B, Cytokine phenotype of T1 and T2 populations. Co-stimulated cells were harvested on day 4 of culture and re-stimulated with anti-CD3/anti-CD28 beads. Resultant 24-hour supernatants were analyzed for cytokine content by enzyme-linked immunosorbent assay. Values shown are mean ± SEM of 3 experiments; cytokine values are expressed as picograms per milliliter per 0.5 × 106 cells per 24 hours. C, Cytolytic capacity of T1 and T2 cells (day 4 of culture). Total lytic potential was evaluated by heteroconjugate assay (left panel). 51Cr-labeled Jurkat cells were targets; assay was performed with or without anti-CD3/anti-TNP bispecific antibody. Non-granule-mediated killing was tested by 51Cr assay in Ca2+-neutralized conditions (right panel). T1 and T2 cells were stimulated with phorbol 12-myristate 13-acetate and calcium ionophore; targets were wild type (L1210) or fas transfected (L1210-fas). CTL results show 1 representative experiment out of 3.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )