Presentation is loading. Please wait.

Presentation is loading. Please wait.

Tissue-engineered esophagus: experimental substitution by onlay patch or interposition  Tracy Grikscheit, MD, Erin R Ochoa, MD, Ashok Srinivasan, PhD,

Published byLeslie Sullivan Modified over 6 years ago

Similar presentations

Presentation on theme: "Tissue-engineered esophagus: experimental substitution by onlay patch or interposition  Tracy Grikscheit, MD, Erin R Ochoa, MD, Ashok Srinivasan, PhD,"— Presentation transcript:

1 Tissue-engineered esophagus: experimental substitution by onlay patch or interposition 
Tracy Grikscheit, MD, Erin R Ochoa, MD, Ashok Srinivasan, PhD, Henning Gaissert, MD, Joseph P Vacanti, MD  The Journal of Thoracic and Cardiovascular Surgery  Volume 126, Issue 2, Pages (August 2003) DOI: /S (03)

2 Figure 1 TEE derived from a neonatal rat pup. (Hematoxylin and eosin; original magnification 10×.) The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

3 Figure 2 TEE derived from an adult rat. (Hematoxylin and eosin; original magnification 10×.) The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

4 Figure 3 TEE from the TEEI group harvested at day 42, including the gastroesophageal junction. A segment of engineered esophageal epithelia (TEE) on the right is seen next to native stomach gastric glands (GG) on the right. (Hematoxylin and eosin; original magnification 10×.) The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

5 Figure 4 Immunohistochemical detection of the antigen α-smooth muscle actin in TEE shows appropriate staining in the muscularis, although it is more sparse than that seen in the native esophagus. (Original magnification 10×.) The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

6 Figure 5 Percentage of preoperative weight per day of TEEP and TEEI animals. Animals either underwent onlay patching to the abdominal esophagus with TEE (TEEP) or complete interposition grafting in the abdominal esophagus (TEEI). After 3 days with nothing by mouth, rats resumed normal laboratory rat chow consumption for the subsequent 42 days. Weights were taken every other day. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

7 Figure 6 TEE OUs labeled with GFP 24 hours after isolation in culture. Resolution is limited by the bright signal, multicellularity, and culture condition. (Original magnification 20×.) The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

8 Figure 7 TEE labeled with GFP after 4 weeks of growth and 2 weeks of anastomosis. (Original magnification 10×.) The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

9 Figure 8 Fluoroscopy in TEEP animals reveals a wide unobstructed lumen. The asterisk indicates the upper margin of the onlay patch. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

10 Figure 9 Fluoroscopy in TEEI animals reveals stenosis at the upper anastomosis (U) and a dilated TEE that flows into the stomach at the lower anastomosis (L). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

11 Figure 10 Gross examination of TEEI construct at harvest. Native esophagus (NE) is seen joining dilated TEE. Visible Prolene sutures that were part of the initial lower anastomosis have been marked with a black pen to show the margin between the TEE and the stomach (S). The duodenum (D) exits the stomach. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

Download ppt "Tissue-engineered esophagus: experimental substitution by onlay patch or interposition  Tracy Grikscheit, MD, Erin R Ochoa, MD, Ashok Srinivasan, PhD,"

Similar presentations

In vitro tissue engineering of a cardiac graft using a degradable scaffold with an extracellular matrix–like topography  Osamu Ishii, MD, Michael Shin,

In vitro tissue engineering of a cardiac graft using a degradable scaffold with an extracellular matrix–like topography  Osamu Ishii, MD, Michael Shin,
In Vitro Fabrication of a Tissue Engineered Human Cardiovascular Patch for Future Use in Cardiovascular Surgery  Chao Yang, MD, Ralf Sodian, MD, Ping.

In Vitro Fabrication of a Tissue Engineered Human Cardiovascular Patch for Future Use in Cardiovascular Surgery  Chao Yang, MD, Ralf Sodian, MD, Ping.
Alejandro Nieponice, MD, Thomas W. Gilbert, Stephen F

Alejandro Nieponice, MD, Thomas W. Gilbert, Stephen F
Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Hajime.

Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Hajime.
Tracheal regeneration: Myth or fact?

Tracheal regeneration: Myth or fact?
Association of CD14+ monocyte-derived progenitor cells with cardiac allograft vasculopathy  Mohamed Salama, MD, PhD, Olena Andrukhova, PhD, Susanne Roedler,

Association of CD14+ monocyte-derived progenitor cells with cardiac allograft vasculopathy  Mohamed Salama, MD, PhD, Olena Andrukhova, PhD, Susanne Roedler,
Definition of proximal anastomosis in the doors study

Definition of proximal anastomosis in the doors study
Gastroesophageal tumor embolization to the popliteal arteries

Gastroesophageal tumor embolization to the popliteal arteries
Mesothelium regeneration on acellular bovine pericardia loaded with an angiogenic agent (ginsenoside Rg1) successfully reduces postsurgical pericardial.

Mesothelium regeneration on acellular bovine pericardia loaded with an angiogenic agent (ginsenoside Rg1) successfully reduces postsurgical pericardial.
Adenocarcinoma arising from cervical esophageal gastric inlet patch

Adenocarcinoma arising from cervical esophageal gastric inlet patch
Experimental noninferiority trial of synthetic small-caliber biodegradable versus stable vascular grafts  Damiano Mugnai, MD, Jean-Christophe Tille, MD,

Experimental noninferiority trial of synthetic small-caliber biodegradable versus stable vascular grafts  Damiano Mugnai, MD, Jean-Christophe Tille, MD,
Tracheal regeneration: Evidence of bone marrow mesenchymal stem cell involvement  Agathe Seguin, MD, PhD, Sonia Baccari, MD, Muriel Holder-Espinasse, MD,

Tracheal regeneration: Evidence of bone marrow mesenchymal stem cell involvement  Agathe Seguin, MD, PhD, Sonia Baccari, MD, Muriel Holder-Espinasse, MD,
Secretory phospholipase A2 is required to produce histologic changes associated with gastroduodenal reflux in a murine model  Ashok Babu, MD, Xianzhong.

Secretory phospholipase A2 is required to produce histologic changes associated with gastroduodenal reflux in a murine model  Ashok Babu, MD, Xianzhong.
Tissue engineering of autologous aorta using a new biodegradable polymer  Dominique Shum-Tim, MD, Ulrich Stock, MD, Jeff Hrkach, PhD, Toshiharu Shinoka,

Tissue engineering of autologous aorta using a new biodegradable polymer  Dominique Shum-Tim, MD, Ulrich Stock, MD, Jeff Hrkach, PhD, Toshiharu Shinoka,
Trachea-guided generation: Déjà vu all over again?

Trachea-guided generation: Déjà vu all over again?
Renal artery stenosis and aneurysms associated with neurofibromatosis

Renal artery stenosis and aneurysms associated with neurofibromatosis
Intrathoracic esophageal replacement by in situ tissue-engineered esophagus  Yuen Nakase, MD, PhD, Tatsuo Nakamura, MD, PhD, Shuichi Kin, MD, PhD, Susumu.

Intrathoracic esophageal replacement by in situ tissue-engineered esophagus  Yuen Nakase, MD, PhD, Tatsuo Nakamura, MD, PhD, Shuichi Kin, MD, PhD, Susumu.
Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material  Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,

Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material  Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,
Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD 

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD 
Behavior of vital and killed autologous pericardium in the descending aorta of sheep  David T. Cheung, PhD, Suk Jung Choo, MD, Albert C. Grobe, MS, Douglas.

Behavior of vital and killed autologous pericardium in the descending aorta of sheep  David T. Cheung, PhD, Suk Jung Choo, MD, Albert C. Grobe, MS, Douglas.

Similar presentations

Ads by Google