1. Virus Removal Experiments with Meta-Hybrid CERA-System
Luisa Ikner (UA), Yasuhiro Matsui (METAWATER), and Chuck Gerba (UA)
RESEARCH PROPOSAL
UA-P01-F2018
STATUS: New proposal
Relationship to Technology Roadmap: Wastewater Reuse (Direct Potable Reuse)
Background:
Virus removal is a vital control point for the direct potable reuse
Current virus log10 reduction credits for membrane filtration:
microfiltration (0 log10), ultrafiltration (1 log10)
To establish log10 reduction credits for virus removal, membrane filtration technologies must:
demonstrate stability and perform consistently
generate a sufficient amount of data under controlled conditions over time for statistical relevance
be validated for virus removal under treatment plant conditions
Approach (continued):
Determine correlations between MS2 and PMMoV removal to assess the feasibility of PMMoV as an indicator of virus removal via membrane filtration
Expected Findings:
Meta-Hybrid CERA-System will achieve consistent MS2 removals of greater than 5 log10
Optimization of the virus concentration methods and qPCR assay will facilitate the use of PMMoV as an indicator of virus removal by the Meta-Hybrid CERA-System
Objectives:
Assess the removal of spiked MS2 virus (surrogate) using the ceramic membrane Meta-Hybrid CERA-System to generate > 20 data sets
Evaluate the use of indigenous Pepper Mild Mottle Virus (PMMoV) as an indicator of human pathogenic virus removal
Budget Requested:
Total $30,000
Approach:
Conduct a minimum of 20 removal evaluations of MS2 and PMMoV per CA Water Resources Control Board recommendations
Analyze MS2 using both infectivity methods (plaque assay method) and molecular qPCR techniques to differentiate between inactivation and physical removal processes
Project Duration:
September 2018 – March 2019
Water & Environmental Technology Center