1. Studies on wheat straw and wood chips degradation in soilBy
Mythreyi Chandoor.

2. Contents
Work accomplished till now
Results and Discussion
New Ideas

3. The basic idea was to incubate the wheat straw and wood chips
Objective :
Main Objective:
The basic idea was to incubate the wheat straw and wood chips
for incubation in Soil to look at its rate of degradation and model the system
and also to find the soil Microcosm responsible for Lignocellulosic Degradation.

4. Work accomplished till now
Soil
Bags filled with wheat straw and wood chips
Pint Jar
Type : Palouse
Moisture : 20%
Carbon : 2.285
Sulphur:
Phosphorous:
Oven dried samples of wheat straw and wood chips
Autoclaved pint jars
Jars = 40 ,a set of 4 for every 4 weeks
incubation temperature = 21 0 centigrade
Control = soil .

5. Serial Dilutions of Soil
Elementary analysis
Serial Dilutions of Soil
Media used : LB and PDA
Wheat straw samples
Media used : LB and PDA
Wood samples
Media used : LB AND PDA

6. PDA + Azure and LBA +Azure
Selective media ,growth on Azo-Dyes
Control
PDA + Azure and LBA +Azure
Overnight incubation
At 30 degrees centigrade

7. Discolouration in Broth media (poly dyes and Azodyes )
sno
Absorbance at 370nm for Poly -R
10-5
0.264
0.257
10-2
0.258
10-1
0.261
STRAW
0.579
0.586
10-6
0.253
10-4
0.270
Poly –R Dye
Dilutions
Absorbance
At 370 nm
100
0.572 10
0.063 1
0.014
0.5
0.004
0.1
0.003
sno
Absorbance at 370nm for Poly -B
10-5
0.585
0.581
10-2
0.273
0.276
10-1
0.664
0.663
STRAW
0.257
0.264
10-6
0.245
0.243
10-4
0.248
Poly –B Dye
Dilutions
Absorbance
At 370 nm
100
0.534
0.281 10
0.181 1
0.024
0.5
0.035
0.1
0.001
sno
Absorbance at 370nm for LB media
10-5
0.369
0.356
10-2
0.390
0.475
10-1
0.384
0.389
STRAW
0.352
10-6
0.364
0.377
10-4
0.351
0.370
LB +azure
Dilutions
Absorbance
At 370 nm
100
0.783 10
0.080 1
0.015
0.5
0.001
0.1
0.000 50
0.343 50
0.281 50
0.407

8. Elementary analysis of Wheat Straw (GC –MS Pyrolysis)
CO2
Elementary analysis of Wheat Straw (GC –MS Pyrolysis)

9. Elementary analysis of Wood Chips (GC –MS Pyrolysis)

10. Pretreatment and Fermentation
New idea !!
Feedstock
Cellulose
Hemicellulose
Lignin
Ratio
Pretreatment and Fermentation
Input : Feedstock
Database1
Feedstock
Database 2
Microcosm/ Thermo chemical combinations.
Database3
Cost efficiency
Output
The Process
cost structure
products