1. Microscopy

2. Lets give you the Scope of things
Microscopes enlarge images of an object
Magnification
Resolution
All scopes vary in power, magnification, and resolution

3. Compound Light Microscope (LM)
Typically used to see cells and small organisms
Specimen mounted to slide
Light passes through the specimen
Limited magnification and resolution of 2,000x
So what's the magnification power you are using?
Eyepiece power x objective power

4. Electron Microscope A beam of electrons passes through the object
TEM- transmission electron microscope
SEM scanning electron microscope magnification (shows 3D image)

6. Microscope Microscope Parts A) Lenses 1. Ocular lenses
Aka: eyepiece
2. Objective lenses
Lenses located on a revolving piece called the nosepiece

7. Microscope Microscope Parts (cont.) A) Lenses (cont.)
To determine how large the microscope is magnifying the object, you must multiply the ocular lens power times the objective lens power
Ocular x Objective = Total Magnification
Ex: 10x x 40x = 400x magnification

8. Microscope Microscope Parts (cont.) B) Stage C) Stage Clips
Platform where the slide is placed for viewing
C) Stage Clips
Metal clips found on the stage that hold the slide in place

9. Microscope Microscope Parts (cont.) D) Light Source E) Diaphragm
Generates the light for viewing
E) Diaphragm
Located under the stage
Contains the iris
Varies the amount of light that passes through the slide and lenses

10. Microscope Microscope Parts (cont.) F) Adjustment Knobs
Used to focus what your are looking at
Are two adjustment knobs:
1. Coarse Adjustment Knob
2. Fine Adjustment Knob
When focusing, always start with the coarse adjustment knob
Once you find the object, bring it into clear focus using the fine adjustment knob

11. Microscope Microscope Parts (cont.) G) Base H) Arm
Provides support for the bottom of the microscope
H) Arm
Provides support for the side of the microscope
When transporting the microscope, always carry with two hands…one on the arm and the other under the base

12. Microscope Focusing the Microscope
Always start with the lowest objective lens
The lowest objective gives the widest field of view (can see the most)
Use the coarse adjustment knob followed by the fine adjustment knob to bring your object into focus
Once in focus, center your object and then change to the next objective lens
Your object should remain in focus (you might have to make some slight adjustments)

13. Microscope Microscope Care Always take care of your microscope
Transport with two hands!
Only clean lenses with lens paper
Tissues, shirt sleeves, paper towels, etc. will scratch the lenses!
Cover with a plastic cover when not using