1. Heat Shock Factor Protein Family of Transcription Factors
By Brian Yu

2. So…What is the question?
Leads to the formation of:

3. What is a Heat Shock Factor Protein?
It is a group of proteins that are formed when in the presence of heat (also seen when there is some stress to the organism)
Found in all biological levels.
Generally, these proteins serve as a way to help other proteins maintain their shape as heat will tend to denature the protein.

5. Genotyping Gels: At5g62020 At4g11660

6. General Information about the two genes:
At5g62020
At4g11660
Gene Size (bp)
1555 bp
2076 bp
# amino acids
300 aa
378 aa
#TDNA inserts (actual) 2
#TDNA inserts (predicted) 1

7. Graphical Interpretation of tDNA insert area
At5g62020
5’ UTR
Exon
Exon
3’ UTR
SALK predicted location (wrong orientation)
TDNA insert 5’ -> 3’
TDNA insert 3’ -> 5’
Exon
Exon
5’ UTR
3’ UTR
At4g11660
SALK predicted location (Wrong orientation)
*not to scale

8. RT-PCR data and GeneChip data for At5g62020

9. Inflorescence
RT PCR Results for At4g11660

10. Promoter Region Experiment:
EcoR1 Digest for pAt4g11660
EcoR1 Digest for pAt5g62020

11. Orientation of the insert in Topo Plasmid (At5g62020)
T7 Site
5’ kB ’
SP6 Site
Promoter Region
Topo Plasmid

12. Any Observable phenotypes in the seed?
Mut
At5g62020
Mut
At4g11660 WT

13. Observable Phenotypic Variations in the seeds?
Mut
At5g62020
Mut
At4g11660 WT

14. The future of the experiment
Sequence the plasmids for the experiment on At4g11660.
Use the plasmids in further experiments with Green Fluorescence Protein to determine where in the promoter area is the “functional code”