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Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington

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Presentation on theme: "Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington"— Presentation transcript:

1 Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices
Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington July 29, 2010 NSF Research Experiences for Undergraduates (REU) in Novel Advanced Materials and Processing with Applications in Biomedical, Electrical and Chemical Engineering

2 Objective Design localized and automated delivery system for microfluidic brain slice devices (μBSDs) Delivers chemicals/drugs and gases Automatically mixes oxygen Characterize delivery of chemicals through tissue Image taken from Mohammed, J, Caicedo, H, Fall, C, & Eddington, D. (2008). Microfluidic add-on for standard electrophysiology chambers. Lab Chip, 8,

3 Motivation μBSDs allow in vitro study of: Neurogenesis Synaptogenesis
Regeneration Protein expression Responses to physical trauma Image taken from Rambani, K, Vukasinovic, J, Glezer, A, & Potter, S. (2009). Culturing thick brain slices: an interstitial 3d microperfusion system for enhanced viability. Journal of Neuroscience Methods, 180,

4 Relevance Chemical Delivery Oxygen Delivery Automation
Spatial resolution Current systems affect entire slice with drug Desire stimulation of specific areas Oxygen Delivery Automatic Mixing Saves money Pre-mixing is not required Automation Multi-tasking Image modified from

5 Oxygen Mixing and Delivery Experimental Set-Up
Y Connector Output Tube Valves Gas Feed Lines: 0% and 21% Oxygen concentration measured by a NeoFox Fiber Optic Oxygen (FOXY) sensor

6 Oxygen Mixing and Delivery Output

7 Oxygen Mixing and Delivery Graphical User Interface (GUI)

8 Delivery Characterization μBSD Design
Reservoir T Channel Delivery Channel

9 Delivery Characterization Experimental Set-up
DI Water Lines T Channel Valve μBSD Vacuum Line

10 Delivery Characterization No Tissue Delivery
10ms 15ms 20ms 25ms 2x5ms 3x5ms 4x5ms 5x5ms

11 Delivery Characterization Linear Relationships: No Tissue
860 Width (µm) 1320 Width (µm)

12 Delivery Characterization Linear Relationships: With Tissue
Full Dose Chemical Delivery Maximum Intensities 10 20 30 Valve Open Time (ms) Intensity Intensity 2 Time (min) Pulsing Dose Chemical Delivery Maximum Intensities 2 4 6 Number of 5ms Pulses Intensity Intensity 2 Time (min)

13 Delivery Characterization GUI

14 Conclusions Oxygen Delivery Chemical Delivery
Precise and accurate results Reliable oxygen switching Chemical Delivery Demonstrated linear relationship Area of effect: ~2mm x 2mm Selective and independent valve control

15 Acknowledgements REU Directors Research Advisor Mentors Funding
NSF-REU DoD-ASSURE EEC-NSF Grant # REU Directors Christos G. Takoudis, Ph.D. Greg Jursich, Ph.D. Research Advisor David Thomas Eddington, Ph.D. Mentors Alexander Blake, Ph.D. Gerardo Mauleon

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