1. The K594ScFv-SEA-D227A fusion protein targets CTL to lyse EA
The K594ScFv-SEA-D227A fusion protein targets CTL to lyse EA.hy926 cells and primary dermal HMVEC. (A) TNF-α-activated EA.hy926 cells (▵) were incubated with K594ScFv-SEA-D227A (0.1 to 10 ng/ml) and CTL. For comparison, unstimulated EA.hy926 cells were included (■).
The K594ScFv-SEA-D227A fusion protein targets CTL to lyse EA.hy926 cells and primary dermal HMVEC. (A) TNF-α-activated EA.hy926 cells (▵) were incubated with K594ScFv-SEA-D227A (0.1 to 10 ng/ml) and CTL. For comparison, unstimulated EA.hy926 cells were included (■). Dermal HMVEC constitutively expressed CD54 (B), and only a slight upregulation was observed upon TNF-α activation (C). HMVEC were negative for CD106 (D) and displayed increased levels when stimulated with TNF-α (E). (F) TNF-α-activated HMVEC were incubated with CTL in the presence of K594ScFv-SEA-D227A (•) and compared to unstimulated cells (□). Experimental conditions were as described in the legend to Fig. 2. The results of one out of two representative experiments are shown.
Kristian Riesbeck et al. Clin. Diagn. Lab. Immunol. 1998; doi: