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A, high resolution MS/MS spectrum (lower panel) of 1435

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1 A, high resolution MS/MS spectrum (lower panel) of 1435
A, high resolution MS/MS spectrum (lower panel) of Da polypeptide indicative for CAD (Table III). A, high resolution MS/MS spectrum (lower panel) of Da polypeptide indicative for CAD (Table III). This peptide was found to be up-regulated in CAD samples compared with control samples (Table I). Swiss-Prot database matching indicated this is a fragment of collagen α-1(I) chain (amino acids 543–558) (Homo sapiens) with a calculated mass of Da and a sequence as indicated (upper panel). B, MS/MS spectrum (lower panel) of Da polypeptide (Table III) up-regulated in CAD samples (Table I). Database matching indicated this is a fragment of collagen α-1(III) chain (amino acids 642–661) (H. sapiens) with a calculated mass of Da and a sequence as indicated (upper panel). The masses of b-ion fragments and y-ion fragments are correlated with the obtained sequence using fragment numbers (52, 53). Abs. Int., absolute intensity. Lukas U. Zimmerli et al. Mol Cell Proteomics 2008;7: © 2008 The American Society for Biochemistry and Molecular Biology

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