1. Embryo incubation and selection in a time-lapse monitoring system improves pregnancy outcome compared with a standard incubator: a retrospective cohort study 
Marcos Meseguer, Ph.D., Irene Rubio, Ph.D., Maria Cruz, Ph.D., Natalia Basile, Ph.D., Julian Marcos, Ph.D., Antonio Requena, M.D. 
Fertility and Sterility 
Volume 98, Issue 6, Pages e10 (December 2012)
DOI: /j.fertnstert
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Differences in clinical pregnancy rate for all intracytoplasmic sperm injection (ICSI) treatments for the period in which the time-lapse monitoring system (TMS) was used in the different clinics: relative improvement in clinical pregnancy rate for all ICSI treatments using TMS versus standard incubator (blue bars, left-hand y-axis), and the number of TMS treatment cycles in each clinic (diamonds, right-hand y-axis). The average value is weighted by the number of TMS cycles in each clinic.
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Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Graphic representation of the two main confounding factors. (A) Oocyte donation: significantly higher pregnancy rate for donation cycles versus autologous cycles and significantly higher abundance of donation cycles in the time-lapse monitoring system. (B) Day of transfer: significantly higher pregnancy rate for day 5 transfer versus day 3 transfer and significantly lower abundance of day 5 transfers in the time-lapse monitoring system.
Fertility and Sterility  , e10DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Evaluation of possible confounding factors to be included in logistic regression model. Only the parameters in the green quadrant are characterized by: 1) a significant effect on pregnancy rate; and 2) a difference in the incidence of each parameter between the time-lapse monitoring system (TMS) and the standard incubator (SI). Those green parameters are categorized as confounding factors and therefore included in the model. The remaining parameters did not present any effect on pregnancy rate (orange) or did not present any different incidence between TMS and SI (red).
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Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 1
Simulated development of accumulated clinical pregnancy rate for three sample clinics. The red line indicates the average pregnancy rate encountered in the standard incubator in the present study (44.9%). The green line is the expected average pregnancy rate assuming a 20.1% relative increase, i.e., 53.9%. The outcome of different clinics would vary initially, but after several hundred cycles the results converge near the expected clinical pregnancy rate. TMS = time-lapse monitoring system.
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Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

6. Supplemental Figure 2
Expected percentage of clinics with a given improvement after a given number of cycles based on a binomial distribution of pregnancy outcome. The clinical pregnancy rate for the standard incubator in the present study was 44.9%. The expectations assume an average pregnancy rate relative increase of 20.1%, i.e., to 53.9%. The different colored areas represent the proportions of clinics that are expected to experience a improvements in pregnancy success rate. With limited number of cycles it is to be expected that many clinics would experience an inferior or an unsustainable elevated result.
Fertility and Sterility  , e10DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

7. Supplemental Figure 3
Simulated development of running average of clinical pregnancy rate for the last 50 cycles for the three sample clinics in Supplemental Figure 1. The red line indicates the average pregnancy rate encountered in the standard incubator in the present study (44.9%). The green line is the expected average pregnancy rate assuming a 20.1% relative increase, i.e., to 53.9%. Given the probability of success, it is expected that all clinics would occasionally experience extended periods with inferior results. TMS = time-lapse monitoring system.
Fertility and Sterility  , e10DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions