1. Serial Dilutions

2. Staple across the bag such that there is space for roots to grow
Get 2 x 3 inch jewelry bags
jewelry supply store.  See product # on second templates/product_list_dro pdown_pix.aspx?GroupGui d=5523 
Staple across the bag such that there is space for roots to grow
Cut piece of paper towel and insert into bag
Add 10 to 15 seeds to each bag

3. Make a 0.2-g/mL NaCl STOCK solution
- Add 2 g of sodium chloride to beaker and add water to 10 mL
0.2 g/mL NaCl
Stock Solution

4. Get as many test tubes (minus two controls) as you have plastic jewelry bags for your experiment

5. Add 5 mL of water to each of your test tubes

6. Add 5 mL of Stock Solution to first test tube.

7. Mix the first Tube

8. Take 5 mL of first tube and add to second tube

9. Mix

10. Repeat and remove 5 ml from last tube and discard

11. Using a 5mL disposable pipette
1. Add 3 mL from stock to a bag
2. Add 3 mL of water to another bag 3. Add the 3 mL from each test tube to
its respective bag

12. Stock Solution

14. Stock

15. Water

16. Water

17. 0.1 g/mL Solution

19. 0.1 g/mL

20. 0.05 g/mL Solution

22. 0.5 g/mL

23. Etc.

24. Let Grow in Dark for ~ 1 week