1. Supplemental Figure 3 A B C T-DNA 1 2 RGLG1 2329bp 3 T-DNA 1 2 RGLG2
...TGATCAAGACGTGTTTAGTTTCTATCC...
...T-DNA/AGACGTGTTTAGTTTCTATCC...
RGLG1
...TAGATGCGATCGTGCAAGCGAGGTTGG...
...T-DNA/CGATCGTGCAAGCGAGGTTGG...
RGLG2 C
WT mutant
100bp
ladder
PCR reaction type
RGLG1
RGLG2
Supplemental Figure 3

2. Supplemental Figure 3. Characterization of T-DNA insertions
in genes RGLG1 and RGLG2. (A) Schematic drawing of
intron-exon structure of the coding regions. Positions of T-DNA
insertion in the mutant alleles used in this work are indicated
by blue arrows. PCR reactions as shown in (C) are indicated
by red numbers, with PCR primers symbolized by black arrows.
(B) Sequences of the transition between genes and T-DNA.
Top, WT sequence, bottom, transition from T-DNA to genomic
sequence. (C) PCR reactions demonstrate absence of full
length transcripts in both cases.
Supplemental Figure 3