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Introduction to Gel Electrophoresis

Published byMalcolm Perkins Modified over 5 years ago

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Presentation on theme: "Introduction to Gel Electrophoresis"— Presentation transcript:

1 Introduction to Gel Electrophoresis

2 What do you remember about DNA?

3 Gel Electrophoresis Gel Electrophoresis - a method for separation and analysis of DNA/protein

4 Separation of Molecules
Separation of molecules is based on size and charge

5 Purpose Studying evolutionary relationships
Forensics: DNA Fingerprinting Paternity Testing

6 How Does it Work? POWER SUPPLY SAMPLE WELLS ELECTROPHORESIS TANK + POSITIVE ELECTRODE DIRECTION OF MOVEMENT -NEGATIVE Samples are placed in a gel, which is placed in a solution that conducts electricity

7 Step 1 Prepare agarose gel

8 Step 2 Prepare DNA samples

9 Step 3 Load samples into gel

10 Step 4 Connect gel to electric current

11 Step 5 Analyze DNA pattern

12 YouTube Gel Electrophoresis

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