1. INTRODUCTION TO LABORATORY OF PLANT PHYSIOLOGY
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INTRODUCTION TO LABORATORY OF PLANT PHYSIOLOGY
Laboratory of Plant Physiology is a very important unit in biological sciences that studies the life of plant
FACULTY OF AGRICULTURE, UNIVERSITY OF BRAWIJAYA
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2. PRESENTATION OUTLINE 1. INTRODUCTION 2. COURSES 3. RESEARCH PRIORITY
1. Definition
2. Scope of Plant Physiology
3. Basic Principle of Crop production
2. COURSES
3. RESEARCH PRIORITY
1. Crop Yield: Productivity (Primary metabolism). Adaptability (Secondary metabolism), and Quality (Primary & Secondary metabolism).
2. Plant Growth
4. LAB. FACILITIES
5. HUMAN RESOURCES
6. JOB OPPORTUNITY
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3. 1. INTRODUCTION 1. Definition
What is the Laboratory of Plant Physiology?
The Lab. Plant physiology is the lab designed to assist students to explore further the life process of plants.
The physical, chemical and biological functions of living plants, right from the whole plant down to the cellular level.
Questions often asked, among others, are: How do plants work:
To produce different organs from single cell/zygote (embryogenesis, cell division & differentiation, and plant growth hormones)
To produce their biomass (photosynthesis & metabolism)
To cope with stress conditions (adaptability/adjustment in primary & secondary metabolism)
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4. 2. Scope of Plant Physiology
Plant physiology has vibrant scope in the field of agriculture.
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5. 3. Basic Principle of Crop production
Source: University of Reading
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6. 2. COURSES
Courses under the responsibility of Plant Physiology Laboratory are:
BOTANY
PLANT BIOCHEMISTRY
PLANT PHYSIOLOGY
PLANT NUTRITION
PLANT GROWTH ANALYSIS
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7. 1. BOTANY 2. PLANT BIOCHEMISTRY 3. PLANT PHYSIOLOGY 4. PLANT NUTRITION
Introduction to plants (cellular level to ecosystem)
2. PLANT BIOCHEMISTRY
The study of life process of plants at molecular level
3. PLANT PHYSIOLOGY
The study of life process of plants (function) at organelle to organ level
4. PLANT NUTRITION
The study of plant nutrition in relation to life process of plants (function)
5. PLANT GROWTH ANALYSIS
The study of plant growth in relation to environment
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8. 3. RESEARCH PRIORITY
1. Crop Yield
εc observed in field experiments: 0.24 (C3) and 0.37 (C4) is far below the theoretical maxima: 0.46 (C3) and 0.6 (C4)
(Flügge et al., 2016)
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9. 2. Plant Growth
This is related to the fact the CROP YIELD (Y) is a function of plant growth: Y = BM x HI
Why the accumulation of plant biomass with time
increases slowly at the beginning
increases rapidly thereafter, and
is almost constant at the end of growing period
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10. BM (biomass production) is determined by photosynthesis
How things happen: What are The Factors determining The Biomass Production ?,. Make it simple, not too complicated

11. Equipments/Instruments
4. LAB. FACILITIES
Examples of Equipment/Instruments in the Lab. Plant Physiology
No.
Equipments/Instruments
Uses* 1.
Microscope binocular
Tissue Analysis 2.
Spectrophotometer (Genesys)
Chemical Analysis 3.
Spectrophotometer (Shimadzu) 4.
HPLC
2nd Metabolite Analysis 5.
LICOR-6400
Photosynthesis 6.
Kjeldahl destilation
Nutrient Analysis 7.
Centrifuge 8.
Strate Porometer
Stomatal Conductance 9.
Hand Refractometer
Sugar Analysis
10.
Chlorophyll meter
Chlorophyll Analysis
11.
Penetrometer
Fruit firmness Analysis
*Lab. Exercises & Research
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12. LICOR-6400
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13. HPLC
HPLC instruments consist of (1) a reservoir of mobile phase, (2) a degassing unit, (3) a pump, (4) an injector, (5) a separation column, and (6) a detector (6).
HPLC Detectors
UV-VIS is most common HPLC detector for a variety of samples such as:
Proteins and peptides
Organic molecules
Pharmaceuticals
Monitor 2 wavelengths at one time
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14. Competency/Research Interest
5. HUMAN RESOURCES
No.
Name
Competency/Research Interest 1.
Prof.Dr.Ir. Tatiek Wardiyati, MS.
Primary metabolites, Potato 2.
Prof. Dr. Ir. S.M. Sitompul
Primary metabolites, Soybean 3.
Prof.Dr.Ir. Titiek Islami, MS.
Primary metabolites, Cassava 4.
Prof.Dr.Ir. Moch.Dawam Maghfoer, MS.
Primary metabolites, Horticulture 5.
Prof.Dr.Ir. Ellis Nihayati, MS.
Secondary metabolites, Tumeric 6.
Dr. Anna Satyana Karyawati , SP.,MP.
Molecular Physiology, Soybean 7.
Dr.agr. Nunun Barunawati, SP., MP.
Plant Nutrition, Tropical Wheat 8.
Ir. Koesriharti , MS.
Horticulture, Vegetables 9.
Mohammad Roviq, SP, MP
Secondary metabolites, Manggo
10.
Wisnu Eko Murdiono , SP., MP.
Secondary metabolites, Vegetables
11.
Deffi Armita, SP.,MS. MP
Secondary metabolites, Roses
12.
Aldila Putri Rahayu, SP.,MP.
Secondary metabolites, Physalis
13.
Rina Megawati
Students’ Laboratory Exercises
14.
Technision (we propose to get one)
Maintenance of Facilities/Research
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15. 6. JOB OPPORTUNITY
1. CONSULTANTS (e.g. functional foods) 2. ENTREPRENEURS (e.g. antioxidants) 3. RESEARCHERS 4. PROFESSORS OTHERS
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17. 4. Recent Advances in Plant Physiology
Review Recent advances in photosynthetic energy conversion
Narendran Sekar, Ramaraja P. Ramasamy
Journal of Photochemistry and Photobiology C: Photochemistry Reviews 22 (2015) 19–33. Journal home page:
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