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IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens  Alexandra F. Santos,

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Presentation on theme: "IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens  Alexandra F. Santos,"— Presentation transcript:

1 IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens  Alexandra F. Santos, MD, MSc, Louisa K. James, PhD, Henry T. Bahnson, MPH, Mohammed H. Shamji, PhD, Natália C. Couto- Francisco, MSc, Sabita Islam, PhD, Sally Houghton, MSc, Andrew T. Clark, FRCPCH, Alick Stephens, PhD, Victor Turcanu, PhD, Stephen R. Durham, MD, FRCP, Hannah J. Gould, PhD, Gideon Lack, MD, FRCPCH  Journal of Allergy and Clinical Immunology  Volume 135, Issue 5, Pages (May 2015) DOI: /j.jaci Copyright © 2015 The Authors Terms and Conditions

2 Fig 1 Peanut-induced activation of mast cells (expressed as a percentage of CD63+ LAD2 cells) sensitized with plasma from patients with PA (n = 6), PS patients (n = 5), and NA subjects (n = 2). Similar results were found for mast cell activation expressed as a percentage of CD107a+ LAD2 cells. Values are presented as means and SEs. P values refer to the comparison between patients with PA and PS patients for each concentration of peanut extract using the Mann-Whitney U test. **P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

3 Fig 2 IgG4/IgE ratios to peanut and peanut allergens in children with PA (n = 65) and PS children (n = 27). Values are presented as medians and interquartile ranges. P values refer to the comparison between children with PA and PS children using the Mann-Whitney U test. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

4 Fig 3 Inhibition of peanut-induced mast cell activation in the presence of plasma from PS patients with detectable P-sIgG4 levels (n = 12) and from patients submitted to POIT (n = 19) but not in the presence of plasma from NA subjects (n = 3). Cells were stimulated with 10 ng/mL peanut extract. Inhibition was tested against the same plasma of a patient with PA. % Inhibition = (% CD63+ cells sensitized with plasma from a patient with PA − % CD63+ cells sensitized with plasma from a patient with PA in the presence of test plasma)/% CD63+ cells sensitized with plasma from a patient with PA. POIT, Patients with PA who underwent POIT (ie, samples collected >6 months after treatment). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

5 Fig 4 Inhibition of peanut-induced activation of mast cells sensitized with plasma from a patient with PA in the presence of mock- or IgG4-depleted plasma samples from peanut-sensitized tolerant patients (median inhibition, 75% vs 30%, respectively; P = .007, n = 12). % Inhibition = (% CD63+ cells sensitized with plasma from a patient with PA − % CD63+ cells sensitized with plasma from a patient with PA in the presence of test plasma)/% CD63+ cells sensitized with plasma from a patient with PA. The P value refers to the comparison between IgG4- and mock-depleted paired samples by using the Wilcoxon signed-rank test. **P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

6 Fig E1 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

7 Fig E2 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

8 Fig E3 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

9 Fig E4 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

10 Fig E5 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

11 Fig E6 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

12 Fig E7 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

13 Fig E8 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

14 Fig E9 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

15 Fig E10 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2015 The Authors Terms and Conditions

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