1. Hyperthyroidism advances luteolysis in the pregnant rat through changes in prostaglandin balance 
Paola B. Navas, Alicia B. Motta, Ph.D., María B. Hapon, Ph.D., Graciela A. Jahn, Ph.D. 
Fertility and Sterility 
Volume 96, Issue 4, Pages e1 (October 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Effect of T4 treatment (A) on serum levels of T3, T4, TSH, PRL, E2, and P on days 19 (G19), 20 (G20), and 21 (G21) of pregnancy and (B) on corpus luteum (CL) number and weight on G21. Thyroxine was administered SC at a concentration of 0.25 mg/kg per day. Results are expressed as the mean ± SEM for groups of eight rats. ∗P<.05 compared with the respective control groups; a,bP<.05 compared with G19 and G20, respectively, in the same treatment group, using two-way analysis of variance and Bonferroni post hoc test.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Effect of T4 treatment on luteal messenger RNA abundances of enzymes related to P synthesis and degradation on days 19 (G19), 20 (G20), and 21 (G21). (A), Measurement by using reverse transcriptase–polymerase chain reaction (RT-PCR) of expression of steroidogenic acute regulatory protein (StAR), cytochrome P450scc, 3βHSD, 20αHSD, and S16. Ethidium bromide fluorescence photograph of the gel electrophoresis of the amplification products. The gel photographs were quantified using NIH Image software and expressed as arbitrary units. (B), Measurement by real-time RT-PCR of the expression of 20αHSD, relative to S16. (C), Western blot of 20αHSD protein on whole luteal protein extracts separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to nitrocellulose and immunoblotted with specific 20αHSD (37 kDa) and α-tubulin (55 kDa) antisera. See Materials and Methods section for further details. Results are expressed as the mean ± SEM of groups of seven to eight rats. ∗P<.05 compared with the respective control groups; a,bP<.05 compared with G19 and G20, respectively, in the same treatment group, using two-way analysis of variance and Bonferroni post hoc test.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Effect of T4 treatment on luteal messenger RNA abundances of proteins involved in the regulation of luteal P synthesis and in prostaglandin synthesis and action on G19, G20, and G21. A, Measurement by using reverse transcriptase–polymerase chain reaction of expression of estrogen receptor alpha (ER-α), estrogen receptor beta (ER-β), PRL-R, PGF2α-R, iNOS, and S16. B, Expression of ER-α, ER-β, PRL-R, PGF2α-R, and iNOS relative to S16, respectively. The films were quantified using NIH Image software and expressed as arbitrary units. Results are expressed as the mean ± SEM of groups of seven to eight rats. See Materials and Methods section for further details. ∗P<.05 compared with the respective control groups; a,bP<.05 compared with G19 in the same treatment group, using two-way analysis of variance and Bonferroni post hoc test.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Effect of T4 treatment on serum concentrations of PGF2α and PGE2 and luteal concentrations of P, PGF2α, and PGE2 on G19, G20, and G21. See Materials and Methods section for further details. Results are expressed as the means ± SEM for groups of seven to eight rats. ∗P<.05 compared with the respective control groups; a,bP<.05 compared with G19 and G20, respectively, in the same treatment group, using two-way analysis of variance and Bonferroni post hoc test.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions