1. You Are My Sunshine

2. Get your strip going (get to step 8 in the Procedure), THEN we’ll talk about the data part. The strips take awhile to develop.

3. Rf value = Distance of band from original spot (mm)
Distance of advancing solvent edge from original spot (mm)
Now, calculate Rf value for Red, Green, and Blue.
Follow the formula.
Advancing Solvent Edge
Blue
Green 56 ? 42 ? 33 ?
Red ? 14
Original spot (pencil line)
2.5 cm

4. Rf values for the practice data
Advancing Solvent Edge?
Red Rf?
Green Rf?
Blue Rf?
(56/56, so Rf is 1)
14 = .25 56
33 = .59 56
42 = .75 56

5. When you measure on your actual strip…
It will probably
look like this:
Etc…
Measure here
At the top of the peak

6. In your data section: Glue strip onto your paper. (Cut in half
<Write Rf formula>
Glue strip onto your
paper. (Cut in half
and share with
your lab partner.)
Cut
Pigment: _____
Rf: ____
Pigment: _____
Rf: ____
Pigment: _____
Rf: ____
Pigment: _____
Rf: ____
Original Spot
Analysis
What: <all colors of the rainbow need to be in one category or the other (see lab)>
Why: <you need to do a little research to see why the above happened>
One constant.
One “what If?”
Etc…

8. Analysis
Analysis
What: <all colors of the rainbow need to be in one category or the other (see lab)>
Why: <you need to do a little research to see why the above happened>
One constant.
One “what If?”
Etc…

9. Here’s what you SHOULD have gotten:
What are some reasons why people might NOT have gotten these Rfs?

10. For Y.A.M.S.
Leaf Colors
The color of a leaf results from an interaction of different pigments produced by the plant. The main pigment classes responsible for leaf color are porphyrins, carotenoids, and flavonoids. The color that we perceive depends on the amount and types of the pigments that are present. Chemical interactions within the plant, particularly in response to acidity (pH) also affect the leaf color.