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Differentiation of Cryptococcus neoformans varieties and Cryptococcus gattii using CAP59-based loop-mediated isothermal DNA amplification  S. Lucas, M.

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Presentation on theme: "Differentiation of Cryptococcus neoformans varieties and Cryptococcus gattii using CAP59-based loop-mediated isothermal DNA amplification  S. Lucas, M."— Presentation transcript:

1 Differentiation of Cryptococcus neoformans varieties and Cryptococcus gattii using CAP59-based loop-mediated isothermal DNA amplification  S. Lucas, M. da Luz Martins, O. Flores, W. Meyer, I. Spencer-Martins, J. Inácio  Clinical Microbiology and Infection  Volume 16, Issue 6, Pages (June 2010) DOI: /j x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

2 FIG. 1 Agarose gel electrophoresis of LAMP products using the primer sets ‘A3', ‘D3' and ‘BC', designed in this work. Reference strains: CN134 (= CBS 887) and CN136 (= CBS 879), C. neoformans var. grubii, serotype A; CN132 (= CBS 888) and WM629 (= CBS 10079), C. neoformans var. neoformans, serotype D; WM178 (= CBS 10082), C. gattii, serotype B; WM779 (= CBS 10101), C. gattii, serotype C; CN135 (= CBS 939) and WM628 (= CBS 10080), hybrids between serotype A and D strains; PYCC 4488T Cryptococcus podzolicus; PYCC 4486T Cryptococcus amylolentus; PYCC 5605, Filobasidiella depauperata; and PYCC 5373T, Tsuchiyaea wingfieldii. M, Molecular Marker GeneRuler° DNA Ladder Mix (Fermentas). Clinical Microbiology and Infection  , DOI: ( /j x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions


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