1. Peptide ID# Pulsed to T2-A24 Cells
Supplemental Figure 1
HLA-A24 Median Fluorescence Intensity
None HIV
XBP1 Unspliced XBP1 Spliced CD CS1
Peptide ID# Pulsed to T2-A24 Cells

2. XBP1 SP223-231 peptide-specific CTL
Supplemental Figure 2
91% 8%
10% 0%
CD3+CD8+
Gated
CD3+CD8-
IFN-g+ Cells
Effector Cells:
XBP1 SP peptide-specific CTL
CD8+
CD8-
CD3+
T cells
SSC
CD3
CD8
IFN-γ
IFN-γ+

3. XBP1 SP223-231 peptide-specific CTL
Supplemental Figure 3
Responder cells:
XBP1 SP peptide-specific CTL
Target cells: None KMS OPM LNCaP
HLA-A24+ XBP HLA-A24- XBP HLA-A24+ XBP1-
6% % % %
17% % %
CTL Proliferation
: Day 5
CFSE low
CFSE low
10% % % %
CTL
Proliferation
: Day 7
CFSE low

4. XBP1 SP223-231 peptide-specific CTL
Supplemental Figure 4
0.3% % %
0.7% % %
% IFN-g+
/CD3+CD8+ T cells
% IL-2+
% CD107a+ IFN-g+
Effector Cells:
XBP1 SP peptide-specific CTL
Target Cells  None HLA-A24+ KMS HLA-A24- OPM1
0.3% % %
IFN-g
CD107a
IL-2
CD8
SSC
IFN-g