1. Instituto de Tecnologia Química e Biológica Universidade Nova de Lisboa The place:

2. OEIRAS The place:

3. Instituto de Tecnologia Química e Biológica Universidade Nova de Lisboa The place: Research divisons: - Chemistry - Biology - Biological chemistry - Technology Supercritical fluid processes

4. The people:Prof. Dr. Manuel Nunes da Ponte Prof. Ana Aguiar Ricardo* Prof. Pedro Simões* Dr. Catarina Duarte Anna Banet Joana Fonseca Rui Ruivo Teresa Casimiro* Vesna Najdanovic-Visak Rita Duarte Ana Serbanovic Raquel Sousa Ana Matias Ana Nunes Post Doc: Ph D students: Junior researchers: *REQUIMTE – Research Center of Excellence

5. The people: Biarritz 2003 Versailles 2003

6. The work – Supercritical Fluid Processes - Extraction from solids - Fraccionation of complex liquid mixtures (liquid – liquid extraction) - Emulsions for protein transport -Chemical Reaction: hydrogenation, oxidation with air, polymerization - Impregnation of polymeric matrixes with pharmaceuticals - Phase Behavior Studies: solubility, VLE, polymer sorption and swelling

7. The work – Supercritical Fluid Processes Extraction from solids Laboratory scale Pilot plant

8. The work – Supercritical Fluid Processes Fraccionation of complex liquid mixtures Emulsions for protein transport Pilot plant

9. The work – Supercritical Fluid Processes Liquid – liquid extraction

10. The work – Supercritical Fluid Processes Chemical Reaction Hydrogenation/Oxidation Polymerization

11. The work – Supercritical Fluid Processes Impregnation of polymeric matrixes with pharmaceuticals

12. The work – Supercritical Fluid Processes Phase Behaviour Studies: Solubility measurements

13. The work – Supercritical Fluid Processes (1) CO 2 compressor; (2) rupture disk; (3) thermostatic water bath; (4) six port sampling HPLC-valve; (5) HPLC-valve; (PT) pressure transducer; (TC) temperature controller; (C) equilibrium cell with sapphire windows; (T) glass trap; (B) expansion calibrated cylinder. Phase Behaviour Studies: vapour-liquid equilibria

14. The work – Supercritical Fluid Processes Phase Behaviour Studies: polymer sorption and swelling

15. The work – Supercritical Fluid Processes Relevant recent publications: - Production of phytosqualene and phytosqualane compounds, useful for e.g. pharmaceutical/cosmetic industries, comprises integral carbon dioxide extraction, purification and optional hydrogenation of saponified and dried residues, DE Patent 19934834 (2001). Inventors: Bus, A. Mueller, P. Swidersky, P. Bondioli, P. Simões, J.A.C. Fernandes - "An apparatus for high-pressure VLE measurements using a static mixer. Results for (CO2 + limonene + citral) and (CO2 + limonene + linalool)" J. Fonseca, P.Simoes, M. Nunes da Ponte J. Supercritical Fluids, 2003, 25, 7-17 -- Supercritical CO 2 - induced Phase Changes in Ionic Liquid + Water + Ethanol Solutions: Application to Biphasic Catalysis V. Najdanovic-Visak, A. Serbanovic, J. M.S.S. Esperança, H. J.R. Guedes,L.P.N. Rebelo, M. Nunes da Ponte ChemPhysChem 2003, 4, 1-6 -- Fractionation of Edible Oil Model Mixtures by Supercritical Carbon Dioxide in a Packed Column – Part II: A Mass Transfer Study, R. Ruivo, M.J. Cebola, P.C. Simões, M. Nunes da Ponte, Ind.Eng.Chem.Res., 2002, 41(9), 2305-2315. - Phase Equilibrium for Capsaicin + Water + Ethanol + Supercritical Carbon Dioxide. C. M. M. Duarte, M. Crew, T. Casimiro, A. Aguiar Ricardo, M. Nunes da Ponte J. Supercritical Fluids, 2002, 22, 87-92

16. The work – Supercritical Fluid Processes On-going projects: - EXTEN-Extraction and encapsulation of natural products Program FAIR-ST8009 (Contract n º CT96-1075; c/ DG XII - UE) - Preparation of controlled release systems for ophthalmic applications, Program Sapiens 2001 (Contract n º POCTI/FCB/38213/2001) - Natural antioxidants (POE – Medida 3.1,Contract: 03/2003/00068/192). - … … …

17. Instituto de Tecnologia Química e Biológica Universidade Nova de Lisboa The place: