Presentation is loading. Please wait.

Presentation is loading. Please wait.

Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non- O139 Vibrio cholerae isolate from China  J. Sun, M. Zhou, Q. Wu, Y.

Similar presentations


Presentation on theme: "Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non- O139 Vibrio cholerae isolate from China  J. Sun, M. Zhou, Q. Wu, Y."— Presentation transcript:

1 Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non- O139 Vibrio cholerae isolate from China  J. Sun, M. Zhou, Q. Wu, Y. Ni  Clinical Microbiology and Infection  Volume 16, Issue 8, Pages (August 2010) DOI: /j x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

2 FIG. 1 Nucleotide sequence of a 2782-bp PCR amplicon of the class 1 integron on pMD354A showing the arr-3, dfrA27 and aadA16 gene cassettes and parts of the 5′-conserved and 3′-conserved segments of the integron. The deduced amino acid sequences of the novel dfrA27 and aadA16 genes are shown below the nucleotide sequence. The start codons of the open reading frames are indicated by horizontal arrows, and the stop codons are indicated by asterisks. The –35 and –10 promoter regions are underlined. The core sites and inverse core sites are boxed. The 102-bp putative promoter-bearing sequences are in bold. The primers are indicated by grey boxes. Clinical Microbiology and Infection  , DOI: ( /j x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

3 FIG. 2 Alignment and boundary structure of the 102-bp promoter-bearing sequences upstream from some aminoglycoside resistance genes and the qacEΔ1 gene. The putative core sites are boxed. The palindrome is indicated by grey boxes. The genes following the promoter sequence are indicated by arrows. Clinical Microbiology and Infection  , DOI: ( /j x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions


Download ppt "Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non- O139 Vibrio cholerae isolate from China  J. Sun, M. Zhou, Q. Wu, Y."

Similar presentations


Ads by Google