1. Organotypic culture methodology reveals normalisation of basal cell polarity and epidermal:dermal junction in pericyte co-cultures.
Organotypic culture methodology reveals normalisation of basal cell polarity and epidermal:dermal junction in pericyte co-cultures. (A) Schematic of OC technique illustrating establishment of 3D skin regeneration within a six-well insert, where cells are grown initially submerged in culture media to establish a DE consisting of fibroblasts, pericytes, or both within a type 1 collagen gel and then maintained at an air–liquid interface to promote epidermal stratification and differentiation. (B) Histological analysis of OCs seeded with either fibroblasts, pericytes, or both on paraffin sections stained with H&E from two independent experiments—first and second row (supporting data shown from a third experiment in Fig 1A) to verify reproducibility of the observation that pericytes induce basal cell polarization in the basal layer of OCs. (C) Transmission electron microscopy analysis of dermo-epidermal junctional region of OCs from two independent OC experiments seeded with either fibroblasts, pericytes, or both, revealing that hemi-desmosome and basement membrane assembly are consistently observed in pericyte cocultures—images are additional data supporting transmission electron microscopy results shown in Fig 2B. Results in this figure and Fig 2B are representative of 18 random fields from two independent biological replicates. Scale bar = 0.5 μm.
Lizhe Zhuang et al. LSA 2018;1:e
© 2018 Zhuang et al.