1. Evaluation of a new 5'-nuclease real-time PCR assay targeting the Toxoplasma gondii AF genomic repeat 
J. Menotti, Y. J-F. Garin, P. Thulliez, M-C. Sérugue, J. Stanislawiak, P. Ribaud, N. de Castro, S. Houzé, F. Derouin 
Clinical Microbiology and Infection 
Volume 16, Issue 4, Pages (April 2010)
DOI: /j x
Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

2. Fig. 1
Evolution of parasite loads under treatment in iterative blood buffy coat (a), whole blood (b) and serum (c) specimens sampled from a cord blood stem cell transplant recipient with cerebral and disseminated toxoplasmosis. The number of tachyzoites per millilitre of sample assessed by the TaqMan-based real-time PCR assay that targets AF DNA sequence is represented as a function of days post transplantation. Treatment with pyrimethamine plus sulfadiazine was started on day 43 post transplantation.
Clinical Microbiology and Infection  , DOI: ( /j x)
Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

3. Fig. 2
Comparison of the Ct values obtained by the TaqMan-based real-time PCR assay that targets AF DNA sequence, the Light-Cycler real-time PCR assay that targets AF DNA sequence and the Light-Cycler real-time PCR assay that targets B1 gene for the iterative blood buffy coat (a), whole blood (b) and serum (c) specimens sampled from the patient whose evolution of parasite loads is represented in Fig. 1. Specimens represented with a Ct value of 45 are specimens for which no amplification was detected after 45 cycles of amplification.
Clinical Microbiology and Infection  , DOI: ( /j x)
Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions