1. p75NTR, TrkA and sortilin expression levels in patients with JIA
p75NTR, TrkA and sortilin expression levels in patients with JIA. (A) mRNA expression levels of TrkA, p75NTR and sortilin in freshly isolated mononuclear cells from peripheral blood of healthy children (CTRL PBMC) and in mononuclear cells from peripheral blood or from synovial fluids of patients with JIA (respectively, JIA PBMC and JIA SFMC) were quantified by real-time PCR analysis.
p75NTR, TrkA and sortilin expression levels in patients with JIA. (A) mRNA expression levels of TrkA, p75NTR and sortilin in freshly isolated mononuclear cells from peripheral blood of healthy children (CTRL PBMC) and in mononuclear cells from peripheral blood or from synovial fluids of patients with JIA (respectively, JIA PBMC and JIA SFMC) were quantified by real-time PCR analysis. JIA PBMC (n=34) and JIA SFMC (n=66) express low TrkA and high p75NTR mRNA levels compared with CTRL PBMC (n=10). Sortilin, a coreceptor for p75NTR essential for proNGF binding, is highly expressed in JIA SFMC (n=19) and expressed in JIA PBMC (n=13) and in CTRL PBMC (n=7). The results are expressed as arbitrary units (AU) after normalisation with the housekeeping gene GAPDH. (B) p75NTR mRNA levels are higher in SFMC than in PBMC of matched patients with JIA (n=18).  (C) Sortilin mRNA levels are higher in SFMC than in PBMC of matched patients with JIA (n=11). (D) Western blot and densitometric analysis of three independent experiments confirmed that p75NTR was the prevalent NGF receptor in JIA mononuclear cells , while TrkA was the most expressed NGF receptor in CTRL PBMC. Sortilin is also highly expressed in JIA mononuclear cells. *p<0.05, **p<0.01, ***p< JIA, juvenile idiopathic arthritis; NGF, nerve growth factor; PBMC, mononuclear cells isolated from peripheral blood; SFMC, mononuclear cells isolated from synovial fluids.
Gaetana Minnone et al. RMD Open 2017;3:e000441
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