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Modified Real-Time PCR for Detecting, Differentiating, and Quantifying Ureaplasma urealyticum and Ureaplasma parvum  Ellen Vancutsem, Oriane Soetens,

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Presentation on theme: "Modified Real-Time PCR for Detecting, Differentiating, and Quantifying Ureaplasma urealyticum and Ureaplasma parvum  Ellen Vancutsem, Oriane Soetens,"— Presentation transcript:

1 Modified Real-Time PCR for Detecting, Differentiating, and Quantifying Ureaplasma urealyticum and Ureaplasma parvum  Ellen Vancutsem, Oriane Soetens, Maria Breugelmans, Walter Foulon, Anne Naessens  The Journal of Molecular Diagnostics  Volume 13, Issue 2, Pages (March 2011) DOI: /j.jmoldx Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Standard curve (in triplicate) from plasmids containing the U. parvum serotype 3 DNA fragment. Correlation coefficient, 0.996; slope, −3.305; intercept, ; PCR efficiency, 100.7%. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Standard curve in triplicate from plasmids containing the U. urealyticum serotype 10 DNA fragment. Correlation coefficient, 0.995; slope, −3.108; intercept, ; PCR efficiency, 109.8%. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4 Figure 3 Comparison of the semiquantitative culture results (xaxis) (133 culture-positive samples) and the quantitative PCR results (y axis). The qPCR results are split into two groups: quantification of U. urealyticum and U. parvum. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

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