1. Fig. 6. Apoptotic MSCs exert in vivo immunosuppression in a TH2-type inflammation model in the absence of cytotoxic cells.
Apoptotic MSCs exert in vivo immunosuppression in a TH2-type inflammation model in the absence of cytotoxic cells. (A) Luc-MSCs were injected into naïve (n = 3) and OVA + MSCs (n = 6) mice 1 hour after the last challenge. One hour later, mice received Z-DEVD-aminoluciferin and were imaged in three independent experiments. White lines separate multiple photographs assembled in the final image. (B) TLS was measured from (A) (mean ± SD). (C) Eighteen hours after MSC infusion, eosinophil infiltration was assessed in the BAL of naïve (n = 3) and those infused with MSCs (n = 3), OVA (n = 6), and OVA + MSCs (n = 6) in two independent experiments. Means ± SD are shown. (D) Eosinophil infiltration (mean ± SD) evaluated in BAL cytospin preparations from OVA-sensitized mice treated with apoMSCs. Groups were as follows: OVA without apoMSCs (n = 6), OVA treated with apoMSCs (1 × 106; n = 7), and naïve mice receiving apoMSCs (1 × 106; n = 2). Results represent the mean ± SD of three independent experiments. Statistics in (B): unpaired t test. Statistics in (C) and (D): one-way ANOVA and Tukey’s multiple comparison test (*P < 0.05).
Antonio Galleu et al., Sci Transl Med 2017;9:eaam7828
Published by AAAS