1. Neuronal Nitric Oxide Synthase Inhibition Reduces Neuronal Apoptosis After Hypothermic Circulatory Arrest 
Elaine E Tseng, Malcolm V Brock, Mary S Lange, Mary E Blue, Juan C Troncoso, Christopher C Kwon, Charles J Lowenstein, Michael V Johnston, William A Baumgartner 
The Annals of Thoracic Surgery 
Volume 64, Issue 6, Pages (December 1997)
DOI: /S (97)

2. Fig. 1
Photomicrographs of hematoxylin-eosin–stained sections of dentate gyrus of hippocampus (original magnification, ×100.) (A) Normal untreated control dog. (B) Dog sacrificed 8 hours after hypothermic circulatory arrest (HCA) (group 2). Apoptotic cells are indicated with an arrow; apoptotic bodies are indicated with an arrowhead. (C) Dog sacrificed 20 hours after HCA. (D) Dog sacrificed 72 hours after HCA.
The Annals of Thoracic Surgery  , DOI: ( /S (97) )

3. Fig. 2
Electron micrograph of the hippocampus showing a normal cell adjacent to an apoptotic cell (arrow). An apoptotic body is indicated with an arrowhead.
The Annals of Thoracic Surgery  , DOI: ( /S (97) )

4. Fig. 3
7-Nitroindazole (7-NI)–reduced apoptosis 8 hours after hypothermic circulatory arrest (HCA) as seen on hematoxylin-eosin–stained sections. Dentate gyrus at high magnification (original magnification, ×100). (A) Normal untreated control dog. (B) Dog subjected to HCA. Apoptosis is marked with an arrow; an apoptotic body is marked with an arrowhead. (C) Dog subjected to HCA treated with 7-NI.
The Annals of Thoracic Surgery  , DOI: ( /S (97) )

5. Fig. 4
7-Nitroindazole (7-NI)–reduced apoptosis 8 hours after hypothermic circulatory arrest (HCA) as seen on terminal deoxynucleotidyltransferase–mediated dUTP-biotin nick end-labeling–stained sections. Dentate gyrus at high magnification (original magnification, ×100). (A) Normal, untreated control dog. (B) Dog subjected to HCA. Apoptosis is marked with an arrow; an apoptotic body is marked with an arrowhead. (C) Dog subjected to HCA and treated with 7-NI.
The Annals of Thoracic Surgery  , DOI: ( /S (97) )

6. Fig. 5
Dentate gyrus of the hippocampus from a dog sacrificed 8 hours after hypothermic circulatory arrest. (A) Photomicrograph of a hematoxylin-eosin–stained section of the entire dentate gyrus (original magnification, ×4.) The structure of the dentate gyrus is noted; apoptosis is not visible at this magnification. (B) Similar magnification of dentate gyrus stained by terminal deoxynucleotidytransferase–mediated dUTP-biotin nick end-labeling (TUNEL) and viewed by dark-field microscopy. The TUNEL-positive apoptotic cells lighted up as white cells against a dark-blue background. (C) High magnification (original magnification, ×40) of dentate gyrus (inset from B) viewed by bright-field microscopy. Apoptotic cells (arrows) stained dark brown, as opposed to normal cells, which stained light blue.
The Annals of Thoracic Surgery  , DOI: ( /S (97) )

7. Fig. 6
Tympanic membrane temperatures during cooling, hypothermic circulatory arrest (HCA), rewarming, and recovery. (CPB = cardiopulmonary bypass; 7NI = 7-nitroindazole.)
The Annals of Thoracic Surgery  , DOI: ( /S (97) )

8. Fig. 7
In vivo measurement of nitric oxide synthase activity as citrulline concentration (μM) over time. (∗∗ = p < 0.05 from baseline; ∗ = p < 0.05 from hypothermic circulatory arrest [HCA] control; CPB = cardiopulmonary bypass; 7NI = 7-nitroindazole.)
The Annals of Thoracic Surgery  , DOI: ( /S (97) )

9. Fig. 8
Quantitative apoptotic score of dogs that underwent hypothermic circulatory arrest (HCA) alone or HCA and 7-nitroindazole (7NI) treatment. (∗ = p < )
The Annals of Thoracic Surgery  , DOI: ( /S (97) )