1. Fig. 6 pDCs infiltrate the skin of BLM-treated mice, and their depletion attenuates skin fibrosis.
pDCs infiltrate the skin of BLM-treated mice, and their depletion attenuates skin fibrosis. pDC depletion was achieved as described in Materials and Methods. (A to C) Representative images of Siglec-H+ cells (red signal; original magnification, ×400) and (D) average (value based on five skin sections with n = 6 to 13 mice per group) in wild-type (WT) mice injected with (A) PBS and (B) with BLM and of (C) CLEC4C-DTR mice injected with DT and BLM. (E) Representative images of Masson’s trichrome–stained skin sections at a ×10 magnification, arrow indicates the area used to quantify skin thickness, and (F) average of skin thickness of WT mice receiving PBS [left panel in (E); open circle in (F)], WT mice injected with DT and treated with BLM [middle panel in (E); closed circle in (F)], and CLEC4C-DTR mice injected with DT and BLM [right panel in (E); closed square in (F)] is shown. (G) Collagen content in the skin expressed as a ratio of collagen per total protein (tot prot.) is shown. (H and I) Representative images of α–smooth muscle actin (α-SMA) immunohistochemistry of the dermis at a ×40 magnification and averages of the number of α-SMA–positive cells per surface area in dermis are shown. (J to L) Relative gene expression of the indicated genes using the NanoString technology. All values represent means ± SEM; n = 6 to 14 mice for each group. Data are cumulative of three independent experiments, and statistical significance was evaluated using a Mann-Whitney U test. *P < 0.05, **P < 0.01, ***P <
Marie Dominique Ah Kioon et al., Sci Transl Med 2018;10:eaam8458
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