1. Both type I and III IFNR signaling are essential for protection against IA.
Both type I and III IFNR signaling are essential for protection against IA. IFNAR−/− (green lines or symbols), IFNLR1−/− (blue lines or symbols), STAT1−/− (orange line), double-deficient IFNAR−/−IFNLR1−/− (purple line or symbols), and WT control (black lines or symbols) B6 (A) and WT Balb/c (B) animals were challenged with 8 × 107 CEA10 Af conidia and monitored for survival. (A and B) Kaplan-Meier survival plot for 10 mice per group analyzed in two independent experiments. (C and D) Representative images of Gomori ammoniacal silver–stained lung sections captured at ×40 magnification. (E) Representative FACS plot of ROS detection in neutrophils derived from WT (dark gray histogram), IFNAR−/−IFNLR1−/− (purple histogram), and p47Phox−/− (light gray histogram) mice. (F) MFI of ROS generation by airway-infiltrating neutrophils at 48 hours after Af infection as analyzed in (E). (G) Pulmonary fungal burden at 48 hours after Af infection. Each symbol in (F) and (G) represents one mouse. Data shown are cumulative of three independent experiments. **P < 0.01; ***P < 0.001; ****P < , calculated by Kruskal-Wallis nonparametric test for multiple comparisons for each knockout group compared with WT control. Statistical analysis was done with Prism software.
Vanessa Espinosa et al. Sci. Immunol. 2017;2:eaan5357
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