1. What is chromatography?
Boardworks A2 Chemistry
Structure Determination
Chromatography is a series of analytical techniques that can be used to separate mixtures of compounds for further use or for analysis.
In all forms of chromatography, a mobile phase moves through or across a stationary phase.
stationary phase – this phase does not move. Compounds in the mixture are attracted to it (adsorbed) and slowed down. Either a solid or a liquid.
mobile phase – this phase moves.
The more soluble compounds in the mixture are carried faster as the mobile phase moves. Either a liquid or a gas.

2. Thin layer chromatography
Boardworks A2 Chemistry
Structure Determination

3. Column chromatography
Boardworks A2 Chemistry
Structure Determination

4. High performance liquid chromatography
Boardworks A2 Chemistry
Structure Determination
High performance liquid chromatography (HPLC) is a development of column chromatography in which the eluent is pumped through the column at high pressure.
pump
injector
This results in better and faster separation than can be achieved in standard column chromatography.
eluent reservoir
data analysis
column
detector
components collected