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Design of clone-specific probes from genome sequences for rapid PCR-typing of outbreak pathogens
E. López-Camacho, Z. Rentero, G. Ruiz-Carrascoso, J.-J. Wesselink, M. Pérez-Vázquez, S. Lusa-Bernal, P. Gómez-Puertas, R.A. Kingsley, P. Gómez-Sánchez, J. Campos, J. Oteo, J. Mingorance Clinical Microbiology and Infection Volume 20, Issue 11, Pages O891-O893 (November 2014) DOI: / Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions
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FIG. 1 End-point PCR with sequence type (ST) -specific primers. Genomic DNA from four clinical isolates was tested with primer pairs specific for target region 4 of ST405 and target region 3 of ST11 and PCR products were analysed by agarose gel electrophoresis. A short (197 bp) region of the 16S rRNA gene was used as a control. Clinical Microbiology and Infection , O891-O893DOI: ( / ) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions
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