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Accurate and Reproducible Gene Expression Profiles from Laser Capture Microdissection, Transcript Amplification, and High Density Oligonucleotide Microarray.

Published byTurid Ellingsen Modified over 5 years ago

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Presentation on theme: "Accurate and Reproducible Gene Expression Profiles from Laser Capture Microdissection, Transcript Amplification, and High Density Oligonucleotide Microarray."— Presentation transcript:

1 Accurate and Reproducible Gene Expression Profiles from Laser Capture Microdissection, Transcript Amplification, and High Density Oligonucleotide Microarray Analysis  Veronica Luzzi, Mamatha Mahadevappa, Rajiv Raja, Janet A. Warrington, Mark A. Watson  The Journal of Molecular Diagnostics  Volume 5, Issue 1, Pages 9-14 (February 2003) DOI: /S (10)60445-X Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Electrophoretic analysis of aRNA targets. One microliter of each cRNA target was analyzed on an RNA LabChip and Agilent bioanalyzer. Lane 1: Molecular weight ladder with marker sizes indicated in base pairs. Lane 2: Input breast cancer total cellular RNA used for target generation. Lanes 3 and 4: Duplicate targets synthesized using “Standard protocol (A)” and starting with 10 μg of total RNA. Lanes 5 and 6: Duplicate cRNA samples generated from 10 ng of total RNA after the first round of amplification using the “RiboAmp Two-Round (D)” protocol. Lanes 7 and 8: Duplicate aRNA samples generated from 10 ng of total RNA after the second round of amplification using the “RiboAmp Two-Round (D)” protocol. To simultaneously visualize cRNA after both first and second rounds of amplification, the signal intensity in each lane has been scaled to itself so that lane-to-lane quantitative comparisons are not valid. Background bands in lanes 5 and 6 are system noise that is detected due to the low specific signal obtained from the first round amplification cRNA product. The Journal of Molecular Diagnostics 2003 5, 9-14DOI: ( /S (10)60445-X) Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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